Testing Data
(Surface Staining of human peripheral blood cells with Mouse anti Human CD162:RPE )
Mouse anti-Human CD162 Monoclonal Antibody | anti-CD162 antibody
Mouse Anti Human CD162: RPE
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.
Use 20ul of the undiluted reagent to label 1x106 cells in 100ul
This product should be stored undiluted.
This product is photosensitive and should be protected from light.
Testing Data
(Surface Staining of human peripheral blood cells with Mouse anti Human CD162:RPE )
Testing Data
(Surface Staining of human peripheral blood cells with Mouse anti Human CD162:RPE )
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD3 and RPE conjugated mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD162 . All experiments were performed on human peripheral blood lymphocytes blocked with 10% human serum, gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
Testing Data
(Figure A. FITC conjugated Mouse anti Human CD3 and RPE conjugated mouse IgG1 isotype control . Figure B. FITC conjugated Mouse anti Human CD3 and RPE conjugated Mouse anti Human CD162 . All experiments were performed on human peripheral blood lymphocytes blocked with 10% human serum, gated on live single cell lymphocytes. Data acquired on the ZE5 Cell Analyzer.)
The most well know function of CD162, when activated, is its role in inflammation in that it slows down leucocytes on the surface of activated epithelium. This occurs when one of its ligands, P-, E- or L- selectin on activated platelets or activated endothelial cells bind to its N-terminal extracellular domain. Upon P-selectin binding, a very long molecule is essentially formed which extends from the activated endothelial cells and leukocyte surface layer, These can also effectively capture circulating myeloid cells. Before a ligand can bind to CD162, the receptor must be postranslationally modified with sialylated, fucosylated O-glycans as well as sulfation at the N-terminal tyrosines (Kappelmayer & Nagy 2017). Mutations which interrupt these post translational modifications prevent ligand binding and has been shown to result in patients suffering from infections of the mucosal membrane and skin (Maly et al. 1996).
The TC2 clone has been used in flow cytometry experiments to examine the effects on CD162 expression levels on the surface of neutrophils upon treatment with histidine-rich glycoprotein which can regulate immunothrombosis and inflammation (Wake et al. 2016).
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Apr 2011]
Uniprot Description
SELPLG: A SLe(x)-type glycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. PSGL1 is critical for the initial leukocyte capture.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 12q24
Cellular Component: membrane; integral to plasma membrane; plasma membrane; integral to membrane
Molecular Function: protein binding; receptor binding
Biological Process: blood coagulation; cell adhesion; leukocyte tethering or rolling; leukocyte adhesive activation; leukocyte migration
Research Articles on CD162
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Product Notes
The CD162 selplg (Catalog #AAA225763) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti Human CD162: RPE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD162 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: Max. Dilution: Neat Use 20ul of the undiluted reagent to label 1x106 cells in 100ul. Researchers should empirically determine the suitability of the CD162 selplg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD162, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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