Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells untreated or treated with Insulin using Rabbit anti DOK2 (pTyr299) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Insulin (100nM) for 10 minutes)
Rabbit anti-Human DOK2 Polyclonal Antibody | anti-DOK2 antibody
Rabbit anti DOK2 (pTyr299)
WB: 1:500-1:2000
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 12 months from date of despatch
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells untreated or treated with Insulin using Rabbit anti DOK2 (pTyr299) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Insulin (100nM) for 10 minutes)
Western Blot (WB)
(Western blot analysis of extracts from Jurkat cells untreated or treated with Insulin using Rabbit anti DOK2 (pTyr299) antibody. Cells were cultured in serum free media for 16-20 hours before treatment with Insulin (100nM) for 10 minutes)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti DOK2 (pTyr299) antibody)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Rabbit anti DOK2 (pTyr299) antibody)
DOK2 is phosphorylated by Bcr-Abl, receptor tyrosine kinases and also cytoplasmIc protein tyrosine kinases (G©rard et al. 2004).
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is constitutively tyrosine phosphorylated in hematopoietic progenitors isolated from chronic myelogenous leukemia (CML) patients in the chronic phase. It may be a critical substrate for p210(bcr/abl), a chimeric protein whose presence is associated with CML. This encoded protein binds p120 (RasGAP) from CML cells. [provided by RefSeq, Jul 2008]
Uniprot Description
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation ().
Research Articles on DOK2
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Product Notes
The DOK2 dok2 (Catalog #AAA225223) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti DOK2 (pTyr299) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DOK2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-Paraffin: 1:50-1:100 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the DOK2 dok2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DOK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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