Testing Data #1
(Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD25 (MBS224436) followed by Goat anti Mouse IgG:FITC (MBS235142))
Mouse CD25 Monoclonal Antibody | anti-CD25 antibody
MOUSE ANTI RABBIT CD25
Applications
Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation
Synonyms
CD25; Monoclonal Antibody; MOUSE ANTI RABBIT CD25; anti-CD25 antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
KEI-alpha1
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0 mg/ml (varies by lot)
Sequence Length
160
Applicable Applications for anti-CD25 antibody
Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200
Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200
Perservative Stabilisers
0.09% Sodium Azide (NaN3)
Preparation
Preparation
Immunogen
Cell membrane preparation of HTLV-1 transformed rabbit T cell line
Fusion Partners
Fusion Partners
Target Species
Rabbit
Preparation and Storage
Store at 4 degree C for one month or at -20 degree C for longer. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD25 (MBS224436) followed by Goat anti Mouse IgG:FITC (MBS235142))
Testing Data #1
(Rabbit peripheral blood lymphocytes stained with Mouse anti Rabbit CD25 (MBS224436) followed by Goat anti Mouse IgG:FITC (MBS235142))
Related Product Information for anti-CD25 antibody
Mouse anti Rabbit CD25 antibody, clone KEI-alpha1 recognizes rabbit CD5, a 55kDa cell surface antigen, otherwise known as the interleukin-2 (IL-2) receptor alpha chain. In rabbits, CD25 is expressed on activated T lymphocytes. Mouse anti Rabbit CD25 antibody, clone KEI-alpha1 is reported to inhibit the binding of IL-2 to its receptor (Kotani et al. 1993).
NCBI and Uniprot Product Information
Uniprot Description
Might be involved in growth regulation, and in myelinization in the peripheral nervous system.
Similar Products
Product Notes
The CD25 pmp22 (Catalog #AAA224436) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD25 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS), Immunoprecipitation (IP). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/100; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD25 pmp22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD25, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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