Rabbit ATG4A Polyclonal Antibody | anti-ATG4A antibody

RABBIT ANTI HUMAN ATG4A

Cat. Num. AAA224293

• Gene Names: ATG4A; APG4A; AUTL2
• Reactivity: Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Applications: Immunoprecipitation
• Synonyms: ATG4A; Polyclonal Antibody; RABBIT ANTI HUMAN ATG4A; anti-ATG4A antibody

• Host: Rabbit
• Reactivity: Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises human ATG4A (Autophagy-related protein 4 homolog A), otherwise known as APG4A/Cysteine protease ATG4A, a cytosolic cysteine protease and member of the peptidase C54 family, predominantly expressed in skeletal muscle and brain, which plays a central role in the cleavage of the C-Terminal region of the target proteins GABARAPL2, MAP1LC3 or GABARAP during autophagy. ; ; This item has been shown to be suitable for use in immunoprecipitation using HEK-293T cell lysates.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 374

• Applicable Applications for anti-ATG4A antibody: Immunoprecipitation (IP)
• Application Notes: Immunoprecipitation: Minimum Dilution: 5; Maximum Dilution: 10ug
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
• Immunogen: KLH conjugated synthetic peptide corresponding to amino acids 100-114 of human ATG4A. The corresponding sequence differs by 2 amino acids in mouse and rat.; Buffer Solution
• Antiserum Preparation: Antiserum to human ATG4A was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-ATG4A antibody

MBS224293 specifically recognises human ATG4A (Autophagy-related protein 4 homolog A), otherwise known as APG4A/Cysteine protease ATG4A, a cytosolic cysteine protease and member of the peptidase C54 family, predominantly expressed in skeletal muscle and brain, which plays a central role in the cleavage of the C-Terminal region of the target proteins GABARAPL2, MAP1LC3 or GABARAP during autophagy.MBS224293 has been shown to be suitable for use in immunoprecipitation using HEK-293T cell lysates.

NCBI and Uniprot Product Information

• NCBI GI #: 30795252
• NCBI GeneID: 115201
• NCBI Accession #: NP_443168.2
• NCBI GenBank Nucleotide #: NM_052936.3
• UniProt Accession #: Q8WYN0; O95534; Q5JYY9; Q5JYZ0; Q86VE5; Q96KQ0; Q96KQ1; A6NCH2; B2RAZ7; D3DUY0
• Molecular Weight: 42,453 Da
• NCBI Official Full Name: cysteine protease ATG4A isoform a
• NCBI Official Synonym Full Names: autophagy related 4A, cysteine peptidase
• NCBI Official Symbol: ATG4A
• NCBI Official Synonym Symbols: APG4A; AUTL2
• NCBI Protein Information: cysteine protease ATG4A; APG4 autophagy 4 homolog A; ATG4 autophagy related 4 homolog A; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase; autophagin 2; autophagin-2; autophagy-related cysteine endopeptidase 2; autophagy-related protein 4 homolog A; hAPG4A
• UniProt Protein Name: Cysteine protease ATG4A
• Protein Family: Cysteine protease
• UniProt Gene Name: ATG4A
• UniProt Synonym Gene Names: APG4A; AUTL2; hAPG4A
• UniProt Entry Name: ATG4A_HUMAN

NCBI Description

Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Transcript variants that encode distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

ATG4A: Cysteine protease required for autophagy, which cleaves the C-terminal part of either MAP1LC3, GABARAPL2 or GABARAP, allowing the liberation of form I. A subpopulation of form I is subsequently converted to a smaller form (form II). Form II, with a revealed C-terminal glycine, is considered to be the phosphatidylethanolamine (PE)-conjugated form, and has the capacity for the binding to autophagosomes. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Widely expressed, at a low level, and the highest expression is observed in skeletal muscle and brain. Also detected in fetal liver. Inhibited by N-ethylmaleimide. Belongs to the peptidase C54 family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.-; Microtubule-binding; Autophagy; Protease

Chromosomal Location of Human Ortholog: Xq22.3

Cellular Component: cytoplasm; cytosol

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: protein delipidation; mitochondrion degradation; C-terminal protein lipidation; protein processing; protein targeting to membrane; proteolysis; autophagic vacuole formation; cellular response to nitrogen starvation