Goat NEUREXIN 1 Polyclonal Antibody | anti-NRXN1 antibody

GOAT ANTI HUMAN NEUREXIN 1 (C-TERMINAL)

Cat. Num. AAA224145

• Gene Names: NRXN1; PTHSL2; SCZD17; Hs.22998
• Reactivity: Dog (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Applications: ELISA, Western Blot
• Synonyms: NEUREXIN 1; Polyclonal Antibody; GOAT ANTI HUMAN NEUREXIN 1 (C-TERMINAL); anti-NRXN1 antibody

• Host: Goat
• Reactivity: Dog (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item specifically recognises an epitope within the C-terminal (CT) region of human Neurexin 1 (NRXN1), a transmembrane neuronal protein and member of the neurexin family, which act as cell adhesion molecules and receptors within the vertebrate nervous system. ; ; NRXN1 exists in many distinct isoforms, the majority of which encode the longer alpha-neurexins generated using an upstream promoter, whilst fewer encode the shorter beta-neurexins, generated using a downstream promoter. Alpha-neurexins have been shown to bind with high affinity to the neuronal protein neurexophilin, whilst neuroligin 1, enriched in synaptic plasma membranes, has been identified as a splice site-specific ligand for beta-neurexins.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 1547

• Applicable Applications for anti-NRXN1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 150kDa in human cerebellum cell lysates ; ELISA: Maximum Dilution: 1/32000; Western Blotting: Minimum Dilution: 0.05; Maximum Dilution: 0.1ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Peptide sequence C-KEKQPSSAKSSNKN from the C-Terminal region of NRXN1 (NP_004792.1; NP_620072.1).; Buffer Solution
• Antiserum Preparation: Antiserum to human NRXN1 (CT) was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-NRXN1 antibody

MBS224145 specifically recognises an epitope within the C-terminal (CT) region of human Neurexin 1 (NRXN1), a transmembrane neuronal protein and member of the neurexin family, which act as cell adhesion molecules and receptors within the vertebrate nervous system. NRXN1 exists in many distinct isoforms, the majority of which encode the longer alpha-neurexins generated using an upstream promoter, whilst fewer encode the shorter beta-neurexins, generated using a downstream promoter. Alpha-neurexins have been shown to bind with high affinity to the neuronal protein neurexophilin, whilst neuroligin 1, enriched in synaptic plasma membranes, has been identified as a splice site-specific ligand for beta-neurexins.

NCBI and Uniprot Product Information

• NCBI GI #: 208609951
• NCBI GeneID: 9378
• NCBI Accession #: NP_001129131.1
• NCBI GenBank Nucleotide #: NM_001135659.1
• UniProt Accession #: Q9ULB1; O60323; Q53TJ9; Q53TQ1; Q9C079; Q9C080; Q9C081; Q9H3M2; Q9UDM6; A7KRL9; P58400
• Molecular Weight: 46,861 Da
• NCBI Official Full Name: neurexin-1-beta isoform alpha2
• NCBI Official Synonym Full Names: neurexin 1
• NCBI Official Symbol: NRXN1
• NCBI Official Synonym Symbols: PTHSL2; SCZD17; Hs.22998
• NCBI Protein Information: neurexin-1-beta; neurexin I
• UniProt Protein Name: Neurexin-1
• Protein Family: Neurexin
• UniProt Gene Name: NRXN1
• UniProt Synonym Gene Names: KIAA0578
• UniProt Entry Name: NRX1A_HUMAN

NCBI Description

Neurexins function in the vertebrate nervous system as cell adhesion molecules and receptors. Two neurexin genes are among the largest known in human (NRXN1 and NRXN3). By using alternate promoters, splice sites and exons, predictions of hundreds or even thousands of distinct mRNAs have been made. Most transcripts use the upstream promoter and encode alpha-neurexin isoforms; fewer transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. Alpha-neurexins contain epidermal growth factor-like (EGF-like) sequences and laminin G domains, and they interact with neurexophilins. Beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins. The RefSeq Project has decided to create only a few representative transcript variants of the multitude that are possible. [provided by RefSeq, Oct 2008]

Uniprot Description

NRXN1: Cell surface protein involved in cell-cell-interactions, excytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)- triggered exocytosis of secretory granules in pituitary gland. They may effect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their calcium-dependent interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latroxin from spider venom. Belongs to the neurexin family. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: presynaptic membrane; cell surface; integral to plasma membrane; endocytic vesicle; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding; metal ion binding; calcium channel regulator activity; acetylcholine receptor binding; receptor activity; calcium ion binding; cell adhesion molecule binding; calcium-dependent protein binding; receptor binding

Biological Process: axon guidance; extracellular matrix organization and biogenesis; prepulse inhibition; neurotransmitter secretion; social behavior; vocal learning; learning; positive regulation of synaptic transmission, glutamatergic; cerebellar granule cell differentiation; negative regulation of filopodium formation; heterophilic cell adhesion; synaptic transmission; positive regulation of synaptogenesis; establishment of protein localization; synaptogenesis; adult behavior; calcium-dependent cell-cell adhesion; neuron adhesion; angiogenesis; neuromuscular process controlling balance

Disease: Pitt-hopkins-like Syndrome 2; Chromosome 2p16.3 Deletion Syndrome