Human NRXN1 (Neurexin-1) ELISA Kit | NRXN1 elisa kit

Human NRXN1 (Neurexin-1) ELISA Kit

Cat. Num. AAA7612157

• Gene Names: NRXN1; PTHSL2; SCZD17; Hs.22998
• Reactivity: Human
• Synonyms: NRXN1 (Neurexin-1); Human NRXN1 (Neurexin-1) ELISA Kit; Neurexin-1; Neurexin I-alpha; Neurexin-1-alpha; NRXN1; KIAA0578; NRXN1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of NRXN1. No significant cross-reactivity or interference between NRXN1 and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.125-5000pg/ml
• Sensitivity: 46.875pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for 6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NRXN1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 208609951
• NCBI GeneID: 9378
• NCBI Accession #: NP_001129131.1
• NCBI GenBank Nucleotide #: NM_001135659.1
• UniProt Accession #: Q9ULB1; O60323; Q53TJ9; Q53TQ1; Q9C079; Q9C080; Q9C081; Q9H3M2; Q9UDM6; A7KRL9; P58400
• Molecular Weight: 46,861 Da
• NCBI Official Full Name: neurexin-1-beta isoform alpha2
• NCBI Official Synonym Full Names: neurexin 1
• NCBI Official Symbol: NRXN1
• NCBI Official Synonym Symbols: PTHSL2; SCZD17; Hs.22998
• NCBI Protein Information: neurexin-1-beta; neurexin I
• UniProt Protein Name: Neurexin-1
• Protein Family: Neurexin
• UniProt Gene Name: NRXN1
• UniProt Synonym Gene Names: KIAA0578
• UniProt Entry Name: NRX1A_HUMAN

NCBI Description

Neurexins function in the vertebrate nervous system as cell adhesion molecules and receptors. Two neurexin genes are among the largest known in human (NRXN1 and NRXN3). By using alternate promoters, splice sites and exons, predictions of hundreds or even thousands of distinct mRNAs have been made. Most transcripts use the upstream promoter and encode alpha-neurexin isoforms; fewer transcripts are produced from the downstream promoter and encode beta-neurexin isoforms. Alpha-neurexins contain epidermal growth factor-like (EGF-like) sequences and laminin G domains, and they interact with neurexophilins. Beta-neurexins lack EGF-like sequences and contain fewer laminin G domains than alpha-neurexins. The RefSeq Project has decided to create only a few representative transcript variants of the multitude that are possible. [provided by RefSeq, Oct 2008]

Uniprot Description

NRXN1: Cell surface protein involved in cell-cell-interactions, excytosis of secretory granules and regulation of signal transmission. Function is isoform-specific. Alpha-type isoforms have a long N-terminus with six laminin G-like domains and play an important role in synaptic signal transmission. Alpha-type isoforms play a role in the regulation of calcium channel activity and Ca(2+)-triggered neurotransmitter release at synapses and at neuromuscular junctions. They play an important role in Ca(2+)- triggered exocytosis of secretory granules in pituitary gland. They may effect their functions at synapses and in endocrine cells via their interactions with proteins from the exocytotic machinery. Likewise, alpha-type isoforms play a role in regulating the activity of postsynaptic NMDA receptors, a subtype of glutamate-gated ion channels. Both alpha-type and beta-type isoforms may play a role in the formation or maintenance of synaptic junctions via their calcium-dependent interactions (via the extracellular domains) with neuroligin family members, CBLN1 or CBLN2. In vitro, triggers the de novo formation of presynaptic structures. May be involved in specification of excitatory synapses. Alpha-type isoforms were first identified as receptors for alpha-latroxin from spider venom. Belongs to the neurexin family. 4 isoforms of the human protein are produced by alternative promoter.

Protein type: Receptor, misc.; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: presynaptic membrane; cell surface; integral to plasma membrane; endocytic vesicle; integral to membrane; plasma membrane; cell junction

Molecular Function: protein binding; metal ion binding; calcium channel regulator activity; acetylcholine receptor binding; receptor activity; calcium ion binding; cell adhesion molecule binding; calcium-dependent protein binding; receptor binding

Biological Process: axon guidance; extracellular matrix organization and biogenesis; prepulse inhibition; neurotransmitter secretion; social behavior; vocal learning; learning; positive regulation of synaptic transmission, glutamatergic; cerebellar granule cell differentiation; negative regulation of filopodium formation; heterophilic cell adhesion; synaptic transmission; positive regulation of synaptogenesis; establishment of protein localization; synaptogenesis; adult behavior; calcium-dependent cell-cell adhesion; neuron adhesion; angiogenesis; neuromuscular process controlling balance

Disease: Pitt-hopkins-like Syndrome 2; Chromosome 2p16.3 Deletion Syndrome