Goat LEPTIN RECEPTOR Polyclonal Antibody | anti-LEPTIN antibody

GOAT ANTI HUMAN LEPTIN RECEPTOR

Cat. Num. AAA222874

• Gene Names: LEPR; OBR; OB-R; CD295; LEP-R; LEPRD
• Reactivity: Dog (Expected from Sequence)
• Applications: ELISA, Western Blot
• Synonyms: LEPTIN RECEPTOR; Polyclonal Antibody; GOAT ANTI HUMAN LEPTIN RECEPTOR; anti-LEPTIN antibody

• Host: Goat
• Reactivity: Dog (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item is specific for human leptin receptor, designated CD295, a type 1 transmembrane glycoprotein and member of the cytokine receptor family, which binds with high affinity to the adipocyte-specific hormone leptin, an obesity factor which regulates adipose-tissue mass/body weight through hypothalamic effects. ; ; The effects of leptin through binding to the long form of CD295 (LRb), are mediated via the JAK/STAT3 signalling pathway, and are vital for the regulation of the normal physiology of many cells, including food intake, lipid metabolism and energy expenditure. CD295-leptin signalling is also involved in the differential regulation of naive and memory T cell proliferation and promotes the release of pro-inflammatory cytokines, including interferon and interleukin-2.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 896

• Applicable Applications for anti-LEPTIN antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 150kDa in human brain cell lysates.; ELISA: Maximum Dilution: 1/16000; Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 3.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Peptide sequence C-TQDDIEKHQSDAG from the internal region of leptin receptor (NP_002294.2; NP_001003679.1; NP_001003680.1).; Buffer Solution
• Antiserum Preparation: Antiserum to human leptin receptor was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-LEPTIN antibody

MBS222874 is specific for human leptin receptor, designated CD295, a type 1 transmembrane glycoprotein and member of the cytokine receptor family, which binds with high affinity to the adipocyte-specific hormone leptin, an obesity factor which regulates adipose-tissue mass/body weight through hypothalamic effects. The effects of leptin through binding to the long form of CD295 (LRb), are mediated via the JAK/STAT3 signalling pathway, and are vital for the regulation of the normal physiology of many cells, including food intake, lipid metabolism and energy expenditure. CD295-leptin signalling is also involved in the differential regulation of naive and memory T cell proliferation and promotes the release of pro-inflammatory cytokines, including interferon and interleukin-2.

NCBI and Uniprot Product Information

• NCBI GI #: 51093379
• NCBI GeneID: 3953
• NCBI Accession #: NP_001003679.1
• NCBI GenBank Nucleotide #: NM_001003679.3
• UniProt Accession #: P48357; Q13592; Q13593; Q13594; Q92919; Q92920; Q92921
• Molecular Weight: 96,042 Da
• NCBI Official Full Name: leptin receptor isoform 3
• NCBI Official Synonym Full Names: leptin receptor
• NCBI Official Symbol: LEPR
• NCBI Official Synonym Symbols: OBR; OB-R; CD295; LEP-R; LEPRD
• NCBI Protein Information: leptin receptor; OB receptor; huB219
• UniProt Protein Name: Leptin receptor
• Protein Family: Leptin receptor
• UniProt Gene Name: LEPR
• UniProt Synonym Gene Names: DB; OBR; LEP-R; OB-R
• UniProt Entry Name: LEPR_HUMAN

NCBI Description

The protein encoded by this gene belongs to the gp130 family of cytokine receptors that are known to stimulate gene transcription via activation of cytosolic STAT proteins. This protein is a receptor for leptin (an adipocyte-specific hormone that regulates body weight), and is involved in the regulation of fat metabolism, as well as in a novel hematopoietic pathway that is required for normal lymphopoiesis. Mutations in this gene have been associated with obesity and pituitary dysfunction. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. It is noteworthy that this gene and LEPROT gene (GeneID:54741) share the same promoter and the first 2 exons, however, encode distinct proteins (PMID:9207021).[provided by RefSeq, Nov 2010]

Uniprot Description

LEPR: the receptor for leptin (obesity factor). A single-transmembrane-domain receptor of the cytokine receptor family. On ligand binding, mediates signaling through JAK2/STAT3. Involved in the regulation of fat metabolism and, in a hematopoietic pathway, required for normal lymphopoiesis. May play a role in reproduction. Five alternatively spliced transcript variants have been reported.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p31

Cellular Component: extracellular region; integral to membrane; plasma membrane; receptor complex

Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; identical protein binding; protein binding; transmembrane receptor activity

Biological Process: cholesterol metabolic process; cell surface receptor linked signal transduction; negative regulation of gluconeogenesis; multicellular organismal development; negative regulation of hydrolase activity; cytokine and chemokine mediated signaling pathway; energy reserve metabolic process

Disease: Leptin Receptor Deficiency