Rabbit anti-Human, Mouse GLYCINE RECEPTOR ALPHA 1/2 Polyclonal Antibody | anti-Glra1 antibody
RABBIT ANTI GLYCINE RECEPTOR ALPHA 1/2 (N-TERMINAL)
Gene Names
Glra1; GLYRA1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Synonyms
GLYCINE RECEPTOR ALPHA 1/2; Polyclonal Antibody; RABBIT ANTI GLYCINE RECEPTOR ALPHA 1/2 (N-TERMINAL); anti-Glra1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Form/Format
Purified
Purified IgG - lyophilised
Purified IgG - lyophilised
Sequence Length
449
Applicable Applications for anti-Glra1 antibody
Immunohistology Frozen, Western Blot (WB)
Application Notes
Western Blot: This item detects a band of approximately 48 kDa in rat spinal cord lysates.
Immunohistology - Frozen: Maximum Dilution: 1/1000
Western Blotting: Maximum Dilution: 1/1000
Immunohistology - Frozen: Maximum Dilution: 1/1000
Western Blotting: Maximum Dilution: 1/1000
Reconstitution
Reconstitute with 0.1 ml sterile PBS Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Buffer Solution
Ammonium bicarbonate
Antiserum Preparation
Antisera to rat glycine receptor (NT) were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Rat
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.
Shelf Life: 12 months from date of reconstitution.
Related Product Information for anti-Glra1 antibody
Rabbit anti glycine receptor recognises an epitope within the N-terminal region (NT) of glycine receptor alpha 1 and alpha 2 subunits. Glycine receptors are members of the ligand-gated ion channel superfamily, which mediate fast inhibitory neurotransmission. The receptors are pentameric membrane proteins which form chloride channels. Binding of glycine to its receptor produces an increase in chloride conductance and membrane hyperpolarisation. Four genes encoding glycine receptor alpha subunits have been identified, together with a single beta polypeptide. Each subunit consists of a large extracellular N-terminal region, four transmembrane domains, and a large cytoplasmic domain.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
51,686 Da
NCBI Official Full Name
glycine receptor subunit alpha-1
NCBI Official Synonym Full Names
glycine receptor, alpha 1
NCBI Official Symbol
Glra1
NCBI Official Synonym Symbols
GLYRA1
NCBI Protein Information
glycine receptor subunit alpha-1; glycine receptor 48 kDa subunit; glycine receptor strychnine-binding subunit; glycine receptor, alpha 1 subunit
UniProt Protein Name
Glycine receptor subunit alpha-1
Protein Family
UniProt Gene Name
Glra1
UniProt Synonym Gene Names
Glyr
UniProt Entry Name
GLRA1_RAT
NCBI Description
glycine receptor strychnine binding subunit; generates glycine gated strychnine sensitive chloride current in the central nervous system [RGD, Feb 2006]
Uniprot Description
Glycine receptors are ligand-gated chloride channels. Channel opening is triggered by extracellular glycine (PubMed:1716350, PubMed:11981023, PubMed:2483325, PubMed:3037383). Channel opening is also triggered by taurine and beta-alanine (PubMed:1716350). Channel characteristics depend on the subunit composition; heteropentameric channels are activated by lower glycine levels and display faster desensitization. Plays an important role in the down-regulation of neuronal excitability. Contributes to the generation of inhibitory postsynaptic currents. Channel activity is potentiated by ethanol (). Potentiation of channel activity by intoxicating levels ot ethanol contribute to the sedative effects of ethanol ().
Research Articles on Glra1
Similar Products
Product Notes
The Glra1 glra1 (Catalog #AAA221817) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI GLYCINE RECEPTOR ALPHA 1/2 (N-TERMINAL) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's GLYCINE RECEPTOR ALPHA 1/2 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Western Blot (WB). Western Blot: This item detects a band of approximately 48 kDa in rat spinal cord lysates. Immunohistology - Frozen: Maximum Dilution: 1/1000 Western Blotting: Maximum Dilution: 1/1000. Researchers should empirically determine the suitability of the Glra1 glra1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GLYCINE RECEPTOR ALPHA 1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
