Goat TROPONIN I Polyclonal Antibody | anti-TNNI3 antibody

GOAT ANTI HUMAN TROPONIN I (CARDIAC)

Cat. Num. AAA221540

• Gene Names: TNNI3; CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• Applications: Immunohistochemistry, ELISA, Immunofluorescence, Western Blot
• Synonyms: TROPONIN I; Polyclonal Antibody; GOAT ANTI HUMAN TROPONIN I (CARDIAC); anti-TNNI3 antibody

• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 210

• Applicable Applications for anti-TNNI3 antibody: Immunohistology Frozen, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Buffer Solution: Phosphate buffered saline
• Antiserum Preparation: Antisera to human cardiac troponin I were raised by repeated immunisations of goats with highly purified antigen.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-TNNI3 antibody

Goat anti Human Troponin I antibody recognizes human cardiac-specific troponin I, (TnI) an integral inhibitory protein of cardiac muscle which exists as a complex with troponin C (TnC) and troponin T (TnT). The TnT subunit of troponin binds to tropomyosin to form a troponin-tropomyosin complex, anchored in place by the binding of TnI to actin, within muscle thin filaments. Structural change resulting from the binding of calcium to specific sites on the regulatory TnC subunit, releases the inhibitory region of TnI from actin, enabling the attachment of the molecular motor protein myosin, allowing for muscle contraction and hence movement. The measurement of blood TnI and TnT levels is an important indicator of heart muscle damage, and can be used to differentiate between angina and myocardic infarction in patients with chest pain. Troponin I has also been shown to inhibit angiogenesis in vivo and in vitro.

NCBI and Uniprot Product Information

• NCBI GI #: 151101270
• NCBI GeneID: 7137
• NCBI Accession #: NP_000354.4
• NCBI GenBank Nucleotide #: NM_000363.4
• UniProt Accession #: P19429
• Molecular Weight: 24,008 Da
• NCBI Official Full Name: troponin I, cardiac muscle
• NCBI Official Synonym Full Names: troponin I type 3 (cardiac)
• NCBI Official Symbol: TNNI3
• NCBI Official Synonym Symbols: CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF
• NCBI Protein Information: troponin I, cardiac muscle; cardiomyopathy, dilated 2A (autosomal recessive)
• UniProt Protein Name: Troponin I, cardiac muscle
• Protein Family: Troponin
• UniProt Gene Name: TNNI3
• UniProt Synonym Gene Names: TNNC1
• UniProt Entry Name: TNNI3_HUMAN

NCBI Description

Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]

Uniprot Description

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Motility/polarity/chemotaxis; Actin-binding; Motor

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: sarcomere; troponin complex; cytosol

Molecular Function: troponin T binding; troponin C binding; protein domain specific binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; protein kinase binding; calcium-dependent protein binding

Biological Process: cellular calcium ion homeostasis; regulation of smooth muscle contraction; heart contraction; heart development; regulation of systemic arterial blood pressure by ischemic conditions; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; muscle filament sliding; cardiac muscle contraction

Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a