Testing Data #1
(Western blot analysis of whole cell lysates from Raji human Burkitt's lymphoma cells probed with Rabbit anti Human CD5L, also known as AIM (MBS221457) at 0.5(A), 1(B) and 2(C) ug/ml)
Rabbit anti-Mouse CD5L Polyclonal Antibody | anti-CD5L antibody
RABBIT ANTI CD5L (C-TERMINAL)
Gene Names
CD5L; AIM; API6; PRO229; Spalpha; SP-ALPHA
Reactivity
Mouse
Applications
Immunohistochemistry, Western Blot
Synonyms
CD5L; Polyclonal Antibody; RABBIT ANTI CD5L (C-TERMINAL); anti-CD5L antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
This item detects an epitope within the C-terminal (CT) region of human CD5L, also known as Apoptosis Inhibitor expressed by Macrophages (AIM), and SP alpha. CD5L is a soluble 38kD glycoprotein which is specifically produced by mature macrophages, and has been shown to support their survival and enhance phagocytic function. CD5L expression is upregulated in response to LXR/RXR heterodimer activation.
CD5L has been shown to mediate the survival of macrophages in atherosclerotic plaques.
CD5L has been shown to mediate the survival of macrophages in atherosclerotic plaques.
Form/Format
Purified
Purified IgG - liquid
Purified IgG - liquid
Concentration
IgG concentration 1.0mg/ml (varies by lot)
Sequence Length
347
Applicable Applications for anti-CD5L antibody
Immunohistology Paraffin*, Western Blot (WB)
Application Notes
Western Blot: This item detects a band of approximately 38kDa in Raji cell lysates.
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.
Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
Perservative Stabilisers
0.02% Sodium Azide
Immunogen
13 amino acid peptide sequence near the carboxy terminus of human CD5L.
Buffer Solution
Buffer Solution
Antiserum Preparation
Antisera to human CD5L were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
Target Species
Human
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Testing Data #1
(Western blot analysis of whole cell lysates from Raji human Burkitt's lymphoma cells probed with Rabbit anti Human CD5L, also known as AIM (MBS221457) at 0.5(A), 1(B) and 2(C) ug/ml)
Testing Data #1
(Western blot analysis of whole cell lysates from Raji human Burkitt's lymphoma cells probed with Rabbit anti Human CD5L, also known as AIM (MBS221457) at 0.5(A), 1(B) and 2(C) ug/ml)
Testing Data #2
(Formalin fixed, paraffin embedded human lymph node stained with Rabbit anti Human CD5L (MBS221457) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Testing Data #2
(Formalin fixed, paraffin embedded human lymph node stained with Rabbit anti Human CD5L (MBS221457) following heat mediated antigen retrieval using sodium citrate buffer (pH6.0))
Related Product Information for anti-CD5L antibody
MBS221457 detects an epitope within the C-terminal (CT) region of human CD5L, also known as Apoptosis Inhibitor expressed by Macrophages (AIM), and SP alpha. CD5L is a soluble 38kD glycoprotein which is specifically produced by mature macrophages, and has been shown to support their survival and enhance phagocytic function. CD5L expression is upregulated in response to LXR/RXR heterodimer activation. CD5L has been shown to mediate the survival of macrophages in atherosclerotic plaques.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,088 Da
NCBI Official Full Name
CD5 antigen-like
NCBI Official Synonym Full Names
CD5 molecule-like
NCBI Official Symbol
CD5L
NCBI Official Synonym Symbols
AIM; API6; PRO229; Spalpha; SP-ALPHA
NCBI Protein Information
CD5 antigen-like; CD5 antigen-like (scavenger receptor cysteine rich family); CT-2; apoptosis inhibitor 6; igM-associated peptide
UniProt Protein Name
CD5 antigen-like
Protein Family
UniProt Gene Name
CD5L
UniProt Synonym Gene Names
API6
UniProt Entry Name
CD5L_HUMAN
Uniprot Description
CD5L: May play a role in the regulation of the immune system. Seems to play a role as an inhibitor of apoptosis.
Protein type: Secreted; Receptor, misc.; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q23.1
Cellular Component: extracellular space; membrane; extracellular region
Molecular Function: scavenger receptor activity
Biological Process: receptor-mediated endocytosis; apoptosis; cellular defense response
Research Articles on CD5L
Similar Products
Product Notes
The CD5L cd5l (Catalog #AAA221457) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The RABBIT ANTI CD5L (C-TERMINAL) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD5L can be used in a range of immunoassay formats including, but not limited to, Immunohistology Paraffin*, Western Blot (WB). Western Blot: This item detects a band of approximately 38kDa in Raji cell lysates. Immunohistology - Paraffin: Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml. Researchers should empirically determine the suitability of the CD5L cd5l for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD5L, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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