Rabbit CD98 LIGHT CHAIN Polyclonal Antibody | anti-CD98 antibody

RABBIT ANTI HUMAN CD98 LIGHT CHAIN (N-TERMINAL)

Cat. Num. AAA221428

• Gene Names: SLC7A5; E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
• Applications: Immunohistochemistry, Western Blot
• Synonyms: CD98 LIGHT CHAIN; Polyclonal Antibody; RABBIT ANTI HUMAN CD98 LIGHT CHAIN (N-TERMINAL); anti-CD98 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects an epitope within the N-terminal (NT) region of the CD98 light chain (CD98LC), also known as the 4F2 light chain, or LAT-1. CD98LC is a 40kD multi-pass membrane protein which forms a heterodimer with the CD98 heavy chain to allow transport of large branched chain, neutral amino acids into the cell. This transport system leads to the uptake of seven amino acids, including isoleucine, phenylalanine, and methionine. ; ; The expression levels of CD98LC are upregulated in a wide spectrum of primary human cancers. Changes in amino acid transporter expression are thought to be an early change in oncogenesis, as they impart growth and survival advantages.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 507

• Applicable Applications for anti-CD98 antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 50-55kDa in human liver cell lysates.; Immunohistology - Paraffin: Minimum Dilution: 15; Maximum Dilution: 25ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 1; Maximum Dilution: 10ug/ml
• Perservative Stabilisers: 0.1% Sodium Azide (NaN3)
• Immunogen: Synthetic peptide: N-terminal region of CD98 light chain; Histology Positive Control Tissue
• Antiserum Preparation: Antisera to CD98 light chain were raised by repeated immunisations of rabbit with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Staining of formalin fixed, paraffin embedded human placenta with Rabbit anti Human CD95 light chain (MBS221428))

Related Product Information for anti-CD98 antibody

MBS221428 detects an epitope within the N-terminal (NT) region of the CD98 light chain (CD98LC), also known as the 4F2 light chain, or LAT-1. CD98LC is a 40kD multi-pass membrane protein which forms a heterodimer with the CD98 heavy chain to allow transport of large branched chain, neutral amino acids into the cell. This transport system leads to the uptake of seven amino acids, including isoleucine, phenylalanine, and methionine. The expression levels of CD98LC are upregulated in a wide spectrum of primary human cancers. Changes in amino acid transporter expression are thought to be an early change in oncogenesis, as they impart growth and survival advantages.

NCBI and Uniprot Product Information

• NCBI GI #: 71979932
• NCBI GeneID: 8140
• NCBI Accession #: NP_003477.4
• NCBI GenBank Nucleotide #: NM_003486.5
• UniProt Accession #: Q01650; Q8IV97; Q9UBN8; Q9UP15; Q9UQC0
• Molecular Weight: 55,010 Da
• NCBI Official Full Name: large neutral amino acids transporter small subunit 1
• NCBI Official Synonym Full Names: solute carrier family 7 (amino acid transporter light chain, L system), member 5
• NCBI Official Symbol: SLC7A5
• NCBI Official Synonym Symbols: E16; CD98; LAT1; 4F2LC; MPE16; hLAT1; D16S469E
• NCBI Protein Information: large neutral amino acids transporter small subunit 1; 4F2 LC; 4F2 light chain; CD98 light chain; L-type amino acid transporter 1; integral membrane protein E16; large neutral amino acids transporter 1; sodium-independent neutral amino acid transporter LAT1; solute carrier family 7 (cationic amino acid transporter, y+ system), member 5; solute carrier family 7 member 5; y+ system cationic amino acid transporter
• UniProt Protein Name: Large neutral amino acids transporter small subunit 1
• UniProt Gene Name: SLC7A5
• UniProt Synonym Gene Names: CD98LC; LAT1; MPE16; 4F2 LC; 4F2LC; hLAT1
• UniProt Entry Name: LAT1_HUMAN

Uniprot Description

SLC7A5: Sodium-independent, high-affinity transport of large neutral amino acids such as phenylalanine, tyrosine, leucine, arginine and tryptophan, when associated with SLC3A2/4F2hc. Involved in cellular amino acid uptake. Acts as an amino acid exchanger. Involved in the transport of L-DOPA across the blood- brain barrier, and that of thyroid hormones triiodothyronine (T3) and thyroxine (T4) across the cell membrane in tissues such as placenta. Plays a role in neuronal cell proliferation (neurogenesis) in brain. Involved in the uptake of methylmercury (MeHg) when administered as the L-cysteine or D,L-homocysteine complexes, and hence plays a role in metal ion homeostasis and toxicity. Involved in the cellular activity of small molecular weight nitrosothiols, via the stereoselective transport of L- nitrosocysteine (L-CNSO) across the transmembrane. May play an important role in high-grade gliomas. Mediates blood-to-retina L- leucine transport across the inner blood-retinal barrier which in turn may play a key role in maintaining large neutral amino acids as well as neurotransmitters in the neural retina. Acts as the major transporter of tyrosine in fibroblasts. Disulfide-linked heterodimer with the amino acid transport protein SLC3A2/4F2hc. Expression induced in quiescent peripheral blood lymphocytes after treatment with phorbol myristate acetate (PMA) and phytohemagglutinin (PHA). Expression and the uptake of leucine is stimulated in mononuclear, cytotrophoblast-like choriocarcinoma cells by combined treatment with PMA and calcium ionophore. Expressed abundantly in adult lung, liver, brain, skeletal muscle, placenta, bone marrow, testis, resting lymphocytes and monocytes, and in fetal liver. Weaker expression in thymus, cornea, retina, peripheral leukocytes, spleen, kidney, colon and lymph node. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Also expressed in all human tumor cell lines tested and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line Caco-2. Belongs to the amino acid-polyamine-organocation (APC) superfamily. L-type amino acid transporter (LAT) (TC 2.A.3.8) family.

Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter; Transporter, SLC family

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: membrane; intracellular membrane-bound organelle; cytoplasm; apical plasma membrane; nucleolus; integral to membrane; plasma membrane; nucleus; cytosol

Molecular Function: L-amino acid transmembrane transporter activity; peptide antigen binding; neutral amino acid transmembrane transporter activity; amino acid transmembrane transporter activity

Biological Process: neutral amino acid transport; amino acid metabolic process; nervous system development; transport; amino acid transport; ion transport; cell differentiation; blood coagulation; leukocyte migration; transmembrane transport