Goat VITAMIN D RECEPTOR Polyclonal Antibody | anti-VDR antibody

GOAT ANTI HUMAN VITAMIN D RECEPTOR

Cat. Num. AAA221386

• Gene Names: VDR; NR1I1; PPP1R163
• Reactivity: Dog (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Applications: ELISA, Western Blot
• Synonyms: VITAMIN D RECEPTOR; Polyclonal Antibody; GOAT ANTI HUMAN VITAMIN D RECEPTOR; anti-VDR antibody

• Host: Goat
• Reactivity: Dog (Expected from Sequence), Mouse (Expected from Sequence), Rat (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item recognises human vitamin D nuclear hormone receptor (VDR) a 48 kDa member of the trans-acting transcriptional regulatory factors. On binding vitamin D, VDR regulates the expression of hormone sensitive genes. The main downstream target of VDR is involved in bone mineral metabolism although VDR is also involved in immune response and cancer metabolic pathways. VDR also acts as a receptor for secondary bile acid lithocholic acid. Additionally, signalling and oncogenic activity of beta-catenin signalling has been shown to be repressed by VDR.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5 mg/ml (varies by lot)
• Sequence Length: 427

• Applicable Applications for anti-VDR antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 40-45kDa in human brain cell lysates. A minimum incubation time of 1 hour is recommended with this antibody.; ELISA: Maximum Dilution: 1/16,000; Western Blotting: Minimum Dilution: 0.3; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide; 0.5% Bovine Serum Albumin
• Immunogen: Peptide sequence CGNQDYKYRVSD corresponding to the internal region of Vitamin D receptor (NP_000367.1).; Buffer Solution
• Antiserum Preparation: Antisera to human Vitamin D receptor were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-VDR antibody

MBS221386 recognises human vitamin D nuclear hormone receptor (VDR) a 48 kDa member of the trans-acting transcriptional regulatory factors. On binding vitamin D, VDR regulates the expression of hormone sensitive genes. The main downstream target of VDR is involved in bone mineral metabolism although VDR is also involved in immune response and cancer metabolic pathways. VDR also acts as a receptor for secondary bile acid lithocholic acid. Additionally, signalling and oncogenic activity of beta-catenin signalling has been shown to be repressed by VDR.

NCBI and Uniprot Product Information

• NCBI GI #: 4507883
• NCBI GeneID: 7421
• NCBI Accession #: NP_000367.1
• NCBI GenBank Nucleotide #: NM_000376.2
• UniProt Accession #: P11473; Q5PSV3; B2R5Q1; G3V1V9
• Molecular Weight: 53,883 Da
• NCBI Official Full Name: vitamin D3 receptor isoform VDRA
• NCBI Official Synonym Full Names: vitamin D (1,25- dihydroxyvitamin D3) receptor
• NCBI Official Symbol: VDR
• NCBI Official Synonym Symbols: NR1I1; PPP1R163
• NCBI Protein Information: vitamin D3 receptor; 1,25-dihydroxyvitamin D3 receptor; nuclear receptor subfamily 1 group I member 1; protein phosphatase 1, regulatory subunit 163; vitamin D nuclear receptor variant 1
• UniProt Protein Name: Vitamin D3 receptor
• Protein Family: Vitamin D3 receptor
• UniProt Gene Name: VDR
• UniProt Synonym Gene Names: NR1I1; VDR
• UniProt Entry Name: VDR_HUMAN

NCBI Description

This gene encodes the nuclear hormone receptor for vitamin D3. This receptor also functions as a receptor for the secondary bile acid lithocholic acid. The receptor belongs to the family of trans-acting transcriptional regulatory factors and shows sequence similarity to the steroid and thyroid hormone receptors. Downstream targets of this nuclear hormone receptor are principally involved in mineral metabolism though the receptor regulates a variety of other metabolic pathways, such as those involved in the immune response and cancer. Mutations in this gene are associated with type II vitamin D-resistant rickets. A single nucleotide polymorphism in the initiation codon results in an alternate translation start site three codons downstream. Alternative splicing results in multiple transcript variants encoding different proteins. [provided by RefSeq, Feb 2011]

Uniprot Description

VDR: Nuclear hormone receptor. Transcription factor that mediates the action of vitamin D3 by controlling the expression of hormone sensitive genes. Regulates transcription of hormone sensitive genes via its association with the WINAC complex, a chromatin-remodeling complex. Recruited to promoters via its interaction with the WINAC complex subunit BAZ1B/WSTF, which mediates the interaction with acetylated histones, an essential step for VDR-promoter association. Plays a central role in calcium homeostasis. Defects in VDR are the cause of rickets vitamin D- dependent type 2A (VDDR2A). A disorder of vitamin D metabolism resulting in severe rickets, hypocalcemia and secondary hyperparathyroidism. Most patients have total alopecia in addition to rickets. Belongs to the nuclear hormone receptor family. NR1 subfamily.

Protein type: DNA-binding; Transcription factor; Nuclear receptor

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: nucleoplasm; nucleus; receptor complex

Molecular Function: retinoid X receptor binding; protein binding; DNA binding; zinc ion binding; sequence-specific DNA binding; steroid hormone receptor activity; vitamin D3 receptor activity; transcription factor activity

Biological Process: lactation; transcription initiation from RNA polymerase II promoter; cell morphogenesis; positive regulation of apoptosis involved in mammary gland involution; negative regulation of transcription from RNA polymerase II promoter; decidualization; signal transduction; intestinal absorption; cellular calcium ion homeostasis; negative regulation of cell proliferation; calcium ion transport; gene expression; steroid hormone mediated signaling; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; skeletal development; positive regulation of keratinocyte differentiation

Disease: Vitamin D-dependent Rickets, Type 2a; Osteoporosis