Goat FTCD Polyclonal Antibody | anti-FTCD antibody

GOAT ANTI HUMAN FTCD (N-TERMINAL)

Cat. Num. AAA221354

• Gene Names: FTCD; LCHC1
• Reactivity: Mouse (Expected from Sequence)
• Applications: ELISA, Western Blot
• Synonyms: FTCD; Polyclonal Antibody; GOAT ANTI HUMAN FTCD (N-TERMINAL); anti-FTCD antibody

• Host: Goat
• Reactivity: Mouse (Expected from Sequence)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects an epitope within the N-terminal (NT) region of formiminotransferase cyclodeaminase (FTCD), also known as 58K Golgi protein. FTCD is a homo-octameric bifunctional enzyme which plays important roles in coupling histidine catabolism with folate metabolism, and integrating the Golgi complex with the vimentin intermediate filament cytoskeleton. ; ; FTCD is present in all mammalian cells either free in the cytosol or tightly bound to the Golgi complex, and is most abundant in the liver. FTCD is linked to two human diseases, autoimmune hepatitis and glutamate formiminotransferase deficiency.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)
• Sequence Length: 158

• Applicable Applications for anti-FTCD antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 55-60kDa in human liver lysate. ; ELISA: Maximum Dilution: 1/64000; Western Blotting: Minimum Dilution: 0.03; Maximum Dilution: 0.5ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3); 0.5% Bovine Serum Albumin
• Immunogen: Peptide with sequence SQLVECVPNFSEGKNQ from the N-terminus of FTCD.; Buffer Solution
• Antiserum Preparation: Antisera to FTCD were raised by repeated immunisations of goat with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-FTCD antibody

MBS221354 detects an epitope within the N-terminal (NT) region of formiminotransferase cyclodeaminase (FTCD), also known as 58K Golgi protein. FTCD is a homo-octameric bifunctional enzyme which plays important roles in coupling histidine catabolism with folate metabolism, and integrating the Golgi complex with the vimentin intermediate filament cytoskeleton. FTCD is present in all mammalian cells either free in the cytosol or tightly bound to the Golgi complex, and is most abundant in the liver. FTCD is linked to two human diseases, autoimmune hepatitis and glutamate formiminotransferase deficiency.

NCBI and Uniprot Product Information

• NCBI GI #: 11140815
• NCBI GeneID: 10841
• NCBI Accession #: NP_006648.1
• NCBI GenBank Nucleotide #: NM_006657.2
• UniProt Accession #: O95954; Q86V03; Q9HCT4; Q9HCT5; Q9HCT6; Q9UHJ2; B9EGD0
• Molecular Weight: 16,503 Da
• NCBI Official Full Name: formimidoyltransferase-cyclodeaminase
• NCBI Official Synonym Full Names: formimidoyltransferase cyclodeaminase
• NCBI Official Symbol: FTCD
• NCBI Official Synonym Symbols: LCHC1
• NCBI Protein Information: formimidoyltransferase-cyclodeaminase; formiminotransferase cyclodeaminase; formiminotransferase-cyclodeaminase
• UniProt Protein Name: Formimidoyltransferase-cyclodeaminase
• Protein Family: Formimidoyltransferase-cyclodeaminase
• UniProt Gene Name: FTCD
• UniProt Synonym Gene Names: FTCD
• UniProt Entry Name: FTCD_HUMAN

NCBI Description

The protein encoded by this gene is a bifunctional enzyme that channels 1-carbon units from formiminoglutamate, a metabolite of the histidine degradation pathway, to the folate pool. Mutations in this gene are associated with glutamate formiminotransferase deficiency. Alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Dec 2009]

Uniprot Description

FTCD: Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool. Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA); also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase; EC 2.1.2.5; Lyase; Amino Acid Metabolism - histidine; Cofactor and Vitamin Metabolism - one carbon pool by folate; EC 4.3.1.4

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: centriole; Golgi apparatus; cytoplasm; cytosol

Molecular Function: formimidoyltetrahydrofolate cyclodeaminase activity; glutamate formimidoyltransferase activity; folic acid binding

Biological Process: folic acid and derivative metabolic process; histidine catabolic process to glutamate and formamide; cytoskeleton organization and biogenesis; histidine catabolic process; histidine catabolic process to glutamate and formate

Disease: Formiminotransferase Deficiency