Testing Data #1
(Staining of mouse spleen cryosection with Rat anti Mouse CD68 (MBS215395) followed by Goat anti Rat IgG:HRP (MBS220363) showing staining of macrophages in the red pulp)
Goat IgG Polyclonal Antibody | anti-IgG antibody
GOAT ANTI RAT IgG (H/L):HRP
Applications
Immunohistochemistry, ELISA, Immunoblot, Western Blot
Synonyms
IgG; Polyclonal Antibody; GOAT ANTI RAT IgG (H/L):HRP; anti-IgG antibody
Host
Goat
Clonality
Polyclonal
Isotype
IgG
Specificity
This item reacts with the heavy and light chains of rat IgG, and the light chains of rat IgA and IgM. Cross reactivity with mouse immunoglobulins has been minimised by cross adsorption with pooled mouse sera and plasmacytoma/hybridoma proteins.
Form/Format
HRP
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid.
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid.
Sequence Length
322
Applicable Applications for anti-IgG antibody
Immunohistology Frozen, ELISA (EIA), Immunoblotting (IB)
Application Notes
ELISA: Minimum Dilution: 1/4000; Maximum Dilution: 1/8000
Perservative Stabilisers
50% Glycerol
Buffer Solution
Phosphate buffered saline
Antiserum Preparation
Antiserum to rat IgG was raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
Target Species
Rat
Preparation and Storage
Store at -20 degree C only. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
Shelf Life: 12 months from date of despatch.
Testing Data #1
(Staining of mouse spleen cryosection with Rat anti Mouse CD68 (MBS215395) followed by Goat anti Rat IgG:HRP (MBS220363) showing staining of macrophages in the red pulp)
Testing Data #1
(Staining of mouse spleen cryosection with Rat anti Mouse CD68 (MBS215395) followed by Goat anti Rat IgG:HRP (MBS220363) showing staining of macrophages in the red pulp)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
35,186 Da
NCBI Official Full Name
Ig gamma-2A chain C region, partial
NCBI Official Symbol
LOC679045
UniProt Protein Name
Ig gamma-2A chain C region
Protein Family
UniProt Gene Name
Igg-2a
UniProt Entry Name
IGG2A_RAT
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Product Notes
The IgG igg-2a (Catalog #AAA220363) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's IgG can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Immunoblotting (IB). ELISA: Minimum Dilution: 1/4000; Maximum Dilution: 1/8000. Researchers should empirically determine the suitability of the IgG igg-2a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IgG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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