Rat anti-Mouse CD206 Monoclonal Antibody | anti-CD206 antibody
RAT ANTI MOUSE CD206
Gene Names
Mrc1; MR; CD206; AW259686
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunohistochemistry, Immunoprecipitation, Immunofluorescence
Purity
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Synonyms
CD206; Monoclonal Antibody; RAT ANTI MOUSE CD206; anti-CD206 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
MR5D3
Specificity
Rat anti mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a ~175 kDa type 1 membrane glycoprotein that is also known as CD206. CD206 is expressed on most tissue macrophages, certain endothelial cells and in vitro derived dendritic cells (Zamze et al. 2002).
The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins (Su et al. 2005).
Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamze et al. 2002). Clone MR5D3 has also been shown to work in Western Blot (Martinez-Pomares et al. 2003 and Su et al. 2005).
The mannose receptor, CD206, is composed of a N-terminal cysteine-rich domain, a fibronectin type II domain, eight tandemly arranged C-type lectin domains (CTLD), a transmembrane domain, and a cytoplasmic domain. The terminal cysteine-rich domain binds sulfated sugars, and the CTLD recognizes carbohydrates terminating in mannose, fucose and N-acetylglucosamine, all sugars found on microorganisms and on some endogenous proteins (Su et al. 2005).
Rat anti mouse CD206 antibody, clone MR5D3 has been reported to be non-inhibitory for the binding of the mannose receptor to carbohydrate ligands (Zamze et al. 2002). Clone MR5D3 has also been shown to work in Western Blot (Martinez-Pomares et al. 2003 and Su et al. 2005).
Purity/Purification
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Form/Format
Purified IgG - liquid
Sequence Length
1456
Applicable Applications for anti-CD206 antibody
Flow Cytometry (FC/FACS)*, Immunohistochemistry - Frozen (IHC-F), Immunoprecipitation (IP), Immunofluorescence (IF)
*CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation.
*CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation.
Application Notes
FC/FACS: 1/10 - 1/20; Use 10ul of the suggested working dilution to label 106 cell in 100ul.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Carrier Free
Yes
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Immunogen
Chimaeric CRD4-7-Fc protein
Fusion Partners
Spleen cells from immunised Fischer rats were fused with cells of the Y3 myeloma cell line
Shelf Life
18 months from date of dispatch.
Preparation and Storage
Store at +4°C or at -20°C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
macrophage mannose receptor 1
NCBI Official Synonym Full Names
mannose receptor, C type 1
NCBI Official Symbol
Mrc1
NCBI Official Synonym Symbols
MR; CD206; AW259686
NCBI Protein Information
macrophage mannose receptor 1; MMR
UniProt Protein Name
Macrophage mannose receptor 1
UniProt Gene Name
Mrc1
UniProt Synonym Gene Names
MMR
UniProt Entry Name
MRC1_MOUSE
Uniprot Description
MRC1: Mediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Receptor, misc.
Cellular Component: cell surface; membrane; plasma membrane; integral to membrane; endosome membrane; endosome
Molecular Function: mannose binding; transmembrane receptor activity; receptor activity; carbohydrate binding
Biological Process: receptor-mediated endocytosis; endocytosis; signal transduction
Research Articles on CD206
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Product Notes
The CD206 mrc1 (Catalog #AAA215657) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The RAT ANTI MOUSE CD206 reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD206 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS)*, Immunohistochemistry - Frozen (IHC-F), Immunoprecipitation (IP), Immunofluorescence (IF) *CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilisation. FC/FACS: 1/10 - 1/20; Use 10ul of the suggested working dilution to label 106 cell in 100ul. Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD206 mrc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD206, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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