Mouse MHC CLASS I Monoclonal Antibody
MOUSE ANTI MOUSE MHC CLASS I:FITC
Applications
Flow Cytometry, Functional Assay
Synonyms
MHC CLASS I; Monoclonal Antibody; MOUSE ANTI MOUSE MHC CLASS I:FITC; anti-MHC CLASS I antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
2G5
Specificity
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. This item recognises a monomorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells. The epitope recognised by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved. Reactivity has been observed with some canine samples suggesting that this antibody may recognise a polymorphic epitope of canine MHC class I.
Form/Format
FITC
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Concentration
IgG concentration 0.1 mg/ml (varies by lot)
Sequence Length
354
Applicable Applications for anti-MHC CLASS I antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5
Perservative Stabilisers
0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation
1% Bovine Serum Albumin
Preparation
Immunogen
Purified H-2Kb and H-2Db MHC I molecules.
Fusion Partners
Fusion Partners
Target Species
Mouse
Preparation and Storage
Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.
Shelf Life: 18 months from date of despatch.
Related Product Information for anti-MHC CLASS I antibody
The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In mice, this complex is referred to as the histocompatibility 2 (H-2) region. MBS212557 recognises a monomorphic epitope present on murine MHC class I molecules, which are expressed at varying levels on the majority of nucleated cells. The epitope recognised by clone 2G5 is conformation dependent and is reported to be phylogenetically conserved.Reactivity has been observed with some canine samples suggesting that this antibody may recognise a polymorphic epitope of canine MHC class I.
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
39,699 Da
NCBI Official Full Name
MHC class I, partial
UniProt Protein Name
MHC class I
Protein Family
UniProt Entry Name
Q95430_PONPY
Uniprot Description
Involved in the presentation of foreign antigens to the immune system.
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Product Notes
The MHC CLASS I (Catalog #AAA215606) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MHC CLASS I can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5. Researchers should empirically determine the suitability of the MHC CLASS I for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC CLASS I, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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