Testing Data
(Staining of permeablised human peripheral blood monocytes with Mouse anti Human CD222:FITC (MBS210828))
Mouse CD222 Monoclonal Antibody | anti-CD222 antibody
MOUSE ANTI HUMAN CD222
Purified IgG - liquid
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/50; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Preparation
Buffer Solution
Shelf Life: 18 months from date of despatch.
Testing Data
(Staining of permeablised human peripheral blood monocytes with Mouse anti Human CD222:FITC (MBS210828))
Testing Data
(Staining of permeablised human peripheral blood monocytes with Mouse anti Human CD222:FITC (MBS210828))
Testing Data
(Staining of permeablised human peripheral blood granulocytes with Mouse anti Human CD222)
Testing Data
(Staining of permeablised human peripheral blood granulocytes with Mouse anti Human CD222)
Testing Data
(Staining of permeablised human peripheral blood granulocytes with Mouse anti Human CD222:APC (MBS215461))
Testing Data
(Staining of permeablised human peripheral blood granulocytes with Mouse anti Human CD222:APC (MBS215461))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate, although the binding sites for either are located on different segments of the receptor. This receptor functions in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. While the related mouse gene shows exclusive expression from the maternal allele, imprinting of the human gene appears to be polymorphic, with only a minority of individuals showing expression from the maternal allele. [provided by RefSeq, Apr 2013]
Uniprot Description
IGF2R: a multifunctional type I transmembrane protein receptor that binds insulin-like growth factor 2 at the cell surface and mannose-6-phosphate-tagged proteins in the trans-Golgi network. Also known as the cation-independent mannose-6 phosphate (M6P) receptor. Clears IGF2 from the cell surface to attenuate signaling, and transports M6P-tagged lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. M6P-tagged lysosomal enzymes bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Belongs to the MRL1/IGF2R family.
Protein type: Receptor, misc.; Vesicle; Membrane protein, integral
Chromosomal Location of Human Ortholog: 6q26
Cellular Component: extracellular space; trans-Golgi network transport vesicle; cell surface; focal adhesion; clathrin coat; membrane; lysosomal membrane; perinuclear region of cytoplasm; endocytic vesicle; integral to plasma membrane; nuclear envelope lumen; late endosome; trans-Golgi network; endosome
Molecular Function: G-protein coupled receptor activity; identical protein binding; mannose binding; protein binding; retinoic acid binding; enzyme binding; transporter activity; G-protein alpha-subunit binding; phosphoprotein binding; receptor activity; glycoprotein binding; insulin-like growth factor II binding; insulin-like growth factor receptor activity
Biological Process: G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; organ regeneration; response to retinoic acid; insulin-like growth factor receptor signaling pathway; positive regulation of apoptosis; spermatogenesis; signal transduction; liver development; post-embryonic development
Disease: Hepatocellular Carcinoma
Research Articles on CD222
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Product Notes
The CD222 igf2r (Catalog #AAA215463) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CD222 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS)*. Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/50; Application Note: Membrane permeabilisation is required for this application. MyBioSource recommends the use of Leucoperm for this purpose. Researchers should empirically determine the suitability of the CD222 igf2r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD222, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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