Mouse ALIX Monoclonal Antibody | anti-ALIX antibody

MOUSE ANTI ALIX

Cat. Num. AAA212843

• Gene Names: PDCD6IP; AIP1; ALIX; HP95; DRIP4
• Applications: ELISA, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot
• Synonyms: ALIX; Monoclonal Antibody; MOUSE ANTI ALIX; anti-ALIX antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3A9
• Form/Format: Purified; Purified IgG - liquid; Phosphate buffered saline
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 271

• Applicable Applications for anti-ALIX antibody: ELISA (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistology Paraffin, Western Blot (WB)
• Application Notes: Immunohistology: This product does not require protein digestion pre-treatment of paraffin sections.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN₃)
• Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
• Species Cross-Reactivity: Human, Mouse, Rat, Xenopus
• Carrier Free: Yes
• Immunogen: Alix-GST fusion Protein
• RRID: AB_872031
• Fusion Partners: Spleen Cells from immunized Balb/c mice were fused with cells of the SP2/0 murine myeloma.
• Preparation and Storage: This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2°C to 8°C for short term use (up to 4 weeks)and store the remaining aliquots at -20°C.; Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

Testing Data

(Western blot analysis of HeLa whole cell lysate probed with MOUSE ANTI ALIX F(ab')2 Rabbit anti Mouse IgG:HRP (MBS235192))

Related Product Information for anti-ALIX antibody

Mouse anti ALIX antibody, clone 3A9 recognizes the apoptosis-linked gene-2 interacting protein X (ALIX), also known as Programmed cell death 6-interacting protein or Hp95. ALIX is a 868 amino acid ~90kDa cytoplasmic protein containing a single BRO1 domain implicated in endosomal targeting (Kim et al. 2005). ALIX was first reported to play a role in apoptosis dependent on its association with PDCD6 (Mahul-Mellier et al. 2006) but has subsequently shown to be involved in endocytosis (Matsuo et al. 2004), viral budding (Irie et al. 2008) and actin cytoskeletal assembly (Pan et al. 2006). Mouse anti Alix, clone 3A9 has been used extensively for the detection of ALIX by Western blotting and for the identification of an ALIX homologue in the sea urchin Paracentrotus lividus (Romancino et al. 2013).

NCBI and Uniprot Product Information

• NCBI GI #: 241982780
• NCBI GeneID: 10015
• NCBI Accession #: NP_001155901.1
• NCBI GenBank Nucleotide #: NM_001162429.2
• UniProt Accession #: Q8WUM4; Q6NUS1; Q9BX86; Q9NUN0; Q9P2H2; Q9UKL5; C5MQH7; E9PFU1
• NCBI Official Full Name: programmed cell death 6-interacting protein isoform 2
• NCBI Official Synonym Full Names: programmed cell death 6 interacting protein
• NCBI Official Symbol: PDCD6IP
• NCBI Official Synonym Symbols: AIP1; ALIX; HP95; DRIP4
• NCBI Protein Information: programmed cell death 6-interacting protein; ALG-2 interacting protein 1; ALG-2-interacting protein X; PDCD6-interacting protein; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4
• UniProt Protein Name: Programmed cell death 6-interacting protein
• UniProt Gene Name: PDCD6IP
• UniProt Synonym Gene Names: AIP1; ALIX; KIAA1375; PDCD6-interacting protein
• UniProt Entry Name: PDC6I_HUMAN

NCBI Description

This gene encodes a protein that functions within the ESCRT pathway in the abscission stage of cytokinesis, in intralumenal endosomal vesicle formation, and in enveloped virus budding. Studies using mouse cells have shown that overexpression of this protein can block apoptosis. In addition, the product of this gene binds to the product of the PDCD6 gene, a protein required for apoptosis, in a calcium-dependent manner. This gene product also binds to endophilins, proteins that regulate membrane shape during endocytosis. Overexpression of this gene product and endophilins results in cytoplasmic vacuolization, which may be partly responsible for the protection against cell death. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. Related pseudogenes have been identified on chromosome 15. [provided by RefSeq, Jan 2012]

Uniprot Description

Alix: an adaptor protein that may regulate the function of receptor and cytoskeleton-associated tyrosine kinases. A class E vacuolar protein sorting (VPS) factor involved in concentration and sorting of cargo proteins of the multivesicular body (MVB) for incorporation into intralumenal vesicles. Fusion between endosomes and the vacuole will then target the cargo proteins to the vacuolar lumen. In case of infection, the HIV-1 virus directly takes advantage of its function for viral exocytosis and budding, via its interaction with HIV-1 p6 protein. May play a role in the regulation of both apoptosis and cell proliferation.

Protein type: Apoptosis; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 3p22.3

Cellular Component: focal adhesion; membrane; cytoplasm; melanosome; microtubule organizing center; immunological synapse; cytosol; vesicle

Molecular Function: protein binding; protein homodimerization activity; proteinase activated receptor binding; SH3 domain binding; calcium-dependent protein binding

Biological Process: protein transport; cell separation during cytokinesis; viral reproduction; apoptosis; viral infectious cycle; mitotic metaphase plate congression; nuclear organization and biogenesis