Mouse CD230 Monoclonal Antibody | anti-CD230 antibody

MOUSE ANTI SHEEP CD230 (PrPSc)

Cat. Num. AAA212818

• Gene Names: PRNP; Prp; SIP; PRPC
• Applications: ELISA, Immunohistochemistry
• Synonyms: CD230; Monoclonal Antibody; MOUSE ANTI SHEEP CD230 (PrPSc); anti-CD230 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 2G11
• Specificity: Transmissible spongiform encephalopathies (TSEs) or prion diseases are fatal infectious neurodegenerative diseases of humans and animals. These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioural changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques. CD230. also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties. This item is specific for PrPSc. This clone does not appear to immunostain the normall cellular PrP expressed in heathly sheep tissue sections resulting in specific labelling of ovine PrPSc. This antibody was raised against a synthetic peptide (146-R154R171-182) of the ovine PrP peptide and specificially recognises the R151-R159 sequence.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0 mg/ml (varies by lot)
• Sequence Length: 256

• Applicable Applications for anti-CD230 antibody: ELISA (EIA), Immunohistology Paraffin*
• Application Notes: ELISA: Tested on peptide. ; ELISA: Minimum Dilution: 1/50; Maximum Dilution: 1/1000; Immunohistology - Paraffin: Application Note: Treatment of tissue sections in 98% formic acid, for 30 minutes, is recommended prior to pre-treatment with trypsin at 37^oC for 5 minutes followed by heat mediated retrieval with 10mM citrate buffer pH6.0 See Andreoletti 2000.
• Perservative Stabilisers: 0.09% Sodium Azide; Preparation
• Immunogen: Synthetic peptide 146-R154R171-182 of ovine PrP; Buffer Solution
• Target Species: Sheep
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-CD230 antibody

Transmissible spongiform encephalopathies (TSEs) or prion diseases are fatal infectious neurodegenerative diseases of humans and animals. These diseases are biologically unique, as they are believed by some to be transmitted by an infectious agent comprised only of protein, with no nucleic acid component. Clinically, these diseases present with motor disturbances and behavioural changes. The major pathological changes seen are neuronal loss, vacuolation (spongiform change), proliferation and branching of glial cells, astrocytic proliferation and accumulation of the prion protein PrPSc, which can form amyloid plaques. CD230. also known as the prion protein (PrP) exists in two alternate forms; a normal cellular form (PrPc) and a disease-associated form (PrPSc).The normal and pathological forms of the prion protein have identical amino acid sequences and differ only in their folded tertiary structure and biochemical properties. MBS212818 is specific for PrPSc. This clone does not appear to immunostain the normall cellular PrP expressed in heathly sheep tissue sections resulting in specific labelling of ovine PrPSc. This antibody was raised against a synthetic peptide (146-R154R171-182) of the ovine PrP peptide and specificially recognises the R151-R159 sequence.

NCBI and Uniprot Product Information

• NCBI GI #: 57164159
• NCBI GeneID: 493887
• NCBI Accession #: NP_001009481.1
• NCBI GenBank Nucleotide #: NM_001009481.1
• UniProt Accession #: P23907; Q5ECG0; Q6V638; Q6V654; Q712W2; Q712W3; Q7JGT4
• Molecular Weight: 27,915 Da
• NCBI Official Full Name: major prion protein
• NCBI Official Symbol: PRNP
• NCBI Official Synonym Symbols: Prp; SIP; PRPC
• NCBI Protein Information: major prion protein; prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia)
• UniProt Protein Name: Major prion protein
• UniProt Gene Name: PRNP
• UniProt Synonym Gene Names: PRP; SIP; PrP
• UniProt Entry Name: PRIO_SHEEP

Uniprot Description

Its primary physiological function is unclear. Has cytoprotective activity against internal or environmental stresses. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu2+ or ZN2+ for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains ().