Intersectin 1 Recombinant Protein | ITSN1 recombinant protein

Recombinant Intersectin 1 (ITSN1)

Cat. Num. AAA2122972

• Gene Names: Itsn1; Ese1; Itsn; Ehsh1; Sh3p17; AA517634; AA545208; AI316805; AI839402; AI848451
• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: Intersectin 1; Recombinant Intersectin 1 (ITSN1); ITSN; SH3D1A; SH3P17; SH3 Domain Protein; Src Homology 3 Domain-Containing Protein; Intersectin 1 Short Form Variant 3; ITSN1 recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Met1~Pro151
• Sequence Length: 248

• Applicable Applications for ITSN1 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Mouse
• Tag: N-terminal His Tag
• Subcellular Location: Secreted
• Isoelectric Point: 6.5
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for ITSN1 recombinant protein

Neuro science

NCBI and Uniprot Product Information

• NCBI GI #: 5731281
• NCBI GeneID: 16443
• UniProt Accession #: Q9Z0R4
• Molecular Weight: Predicted Molecular Mass: 19.8kDa; Accurate Molecular Mass: 20kDa
• NCBI Official Full Name: Ese1 protein, partial
• NCBI Official Synonym Full Names: intersectin 1 (SH3 domain protein 1A)
• NCBI Official Symbol: Itsn1
• NCBI Official Synonym Symbols: Ese1; Itsn; Ehsh1; Sh3p17; AA517634; AA545208; AI316805; AI839402; AI848451
• NCBI Protein Information: intersectin-1
• UniProt Protein Name: Intersectin-1
• Protein Family: Intersectin
• UniProt Gene Name: Itsn1
• UniProt Synonym Gene Names: Ese1; Itsn
• UniProt Entry Name: ITSN1_MOUSE

Uniprot Description

ITSN1: Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2. Isoform 1 could be involved in brain-specific synaptic vesicle recycling. Inhibits ARHGAP31 activity toward RAC1. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding; GEFs; Adaptor/scaffold; GEFs, Rac/Rho

Cellular Component: neuron projection; cell projection; membrane; lamellipodium; endocytic vesicle; plasma membrane; terminal button; synapse; coated pit; cell junction

Molecular Function: protein binding; Rho guanyl-nucleotide exchange factor activity; guanyl-nucleotide exchange factor activity; metal ion binding; kinase activator activity; protein complex scaffold; calcium ion binding

Biological Process: positive regulation of catalytic activity; positive regulation of protein kinase B signaling cascade; small GTPase mediated signal transduction; regulation of Rho protein signal transduction; endocytosis; negative regulation of neuron apoptosis; positive regulation of phosphorylation