Activated Protein C Recombinant Protein | APC recombinant protein

Recombinant Activated Protein C (APC)

Cat. Num. AAA2122696

• Gene Names: APC; GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46
• Applications: SDS-Page, Western Blot
• Purity: >90%
• Synonyms: Activated Protein C; Recombinant Activated Protein C (APC); APC recombinant protein

• Host: E Coli
• Purity/Purification: >90%
• Form/Format: Freeze-dried powder; PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Sequence Positions: Thr2635~Val2843
• Sequence Length: 2843

• Applicable Applications for APC recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Source: Prokaryotic expression
• Species: Human
• Tag: N-terminal His Tag
• Subcellular Location: Cytoplasm
• Isoelectric Point: 9.4
• Reconstitution: Reconstitute in PBS or others.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Product Categories/Family for APC recombinant protein

Enzyme & Kinase; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 53759122
• NCBI GeneID: 324
• NCBI Accession #: NP_000029.2
• NCBI GenBank Nucleotide #: NP_000029.2
• UniProt Accession #: P25054
• Molecular Weight: Predicted Molecular Mass: 26kDa; Accurate Molecular Mass: 33kDa
• NCBI Official Full Name: adenomatous polyposis coli protein isoform b
• NCBI Official Synonym Full Names: APC regulator of WNT signaling pathway
• NCBI Official Symbol: APC
• NCBI Official Synonym Symbols: GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46
• NCBI Protein Information: adenomatous polyposis coli protein
• UniProt Protein Name: Adenomatous polyposis coli protein
• Protein Family: Adenomatous polyposis coli
• UniProt Gene Name: APC
• UniProt Synonym Gene Names: DP2.5; Protein APC
• UniProt Entry Name: APC_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Mutations in the APC gene have been found to occur in most colorectal cancers. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Dec 2019]

Uniprot Description

APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Tumor suppressor

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: centrosome; tight junction; cytosol; beta-catenin destruction complex; nucleoplasm; kinetochore; cell-cell adherens junction; lamellipodium; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus; lateral plasma membrane

Molecular Function: microtubule plus-end binding; protein binding; cadherin binding; gamma-catenin binding; microtubule binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding

Biological Process: positive regulation of cell adhesion; somatic stem cell maintenance; apoptosis; positive regulation of apoptosis; positive regulation of microtubule polymerization; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; cytoplasmic microtubule organization and biogenesis; muscle maintenance; anterior/posterior pattern formation; negative regulation of cell proliferation; regulation of osteoclast differentiation; hair follicle development; cytokinesis after mitosis; mitotic cell cycle spindle assembly checkpoint; positive regulation of epithelial cell differentiation; protein complex assembly; cell adhesion; cell cycle arrest; kidney development; proximal/distal pattern formation; cell structure disassembly during apoptosis; regulation of microtubule-based process; regulation of attachment of spindle microtubules to kinetochore; negative regulation of odontogenesis; cell migration; thymus development; regulation of osteoblast differentiation; negative regulation of MAPKKK cascade; negative regulation of microtubule depolymerization; axis specification; negative regulation of cyclin-dependent protein kinase activity; positive regulation of pseudopodium formation; axonogenesis; dorsal/ventral pattern formation; retina development in camera-type eye; positive regulation of cell division; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; response to DNA damage stimulus; mitotic metaphase/anaphase transition; positive regulation of cell migration

Disease: Familial Adenomatous Polyposis 1; Gastric Cancer; Desmoid Disease, Hereditary; Colorectal Cancer; Hepatocellular Carcinoma