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Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Rat Small intestine Tissue; Primary Ab: 10ug/ml Mouse Anti-Rat HRG AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

Mouse anti-Rat Histidine Rich Glycoprotein (HRG) Monoclonal Antibody | anti-HRG antibody

Monoclonal Antibody to Histidine Rich Glycoprotein (HRG)

Gene Names
LOC681544; Hrg; RNHRG2
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Histidine Rich Glycoprotein (HRG); Monoclonal Antibody; Monoclonal Antibody to Histidine Rich Glycoprotein (HRG); Hematology; anti-HRG antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Clone Number
C1
Specificity
The antibody is a mouse monoclonal antibody raised against HRG. It has been selected for its ability to recognize HRG in immunohistochemical staining and western blotting.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Liquid; PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
1mg/mL (varies by lot)
Sequence
Leu19~Lys525
Sequence Length
515
Applicable Applications for anti-HRG antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes
WB: 0.2-2ug/mL; 1:500-5000
IHC: 5-20ug/mL; 1:50-200
ICC: 5-20ug/mL; 1:50-200
Conjugation
None
Cross Reactivity
Rat
Preparation and Storage
Store at 4 degree C for frequent use. Aliquot and store at -20 degree C for 12 months.
Avoid repeated freeze/thaw cycles.

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Rat Small intestine Tissue; Primary Ab: 10ug/ml Mouse Anti-Rat HRG AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

Immunohistochemistry (IHC) (DAB staining on IHC-P;Samples: Rat Small intestine Tissue; Primary Ab: 10ug/ml Mouse Anti-Rat HRG AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
similar to histidine-rich glycoprotein
NCBI Official Synonym Full Names
similar to histidine-rich glycoprotein
NCBI Official Symbol
LOC681544
NCBI Official Synonym Symbols
Hrg; RNHRG2
NCBI Protein Information
histidine-rich glycoprotein-like; similar to histidine-rich glycoprotein
UniProt Protein Name
Histidine-rich glycoprotein
UniProt Gene Name
Hrg
UniProt Synonym Gene Names
Hrg1; HPRG; HRG1

Uniprot Description

Plasma glycoprotein that binds a number of ligands such as heme, heparin, heparan sulfate, thrombospondin, plasminogen, and divalent metal ions. Inhibits rosette formation. Acts as an adapter protein and implicated in regulating many processes such as immune complex and pathogen clearance, cell adhesion, angiogenesis, coagulation and fibrinolysis. Mediates clearance of necrotic cells through enhancing the phagocytosis of necrotic cells in a heparan sulfate-dependent pathway. This process can be regulated by the presence of certain HRG ligands such as heparin and zinc ions. Binds to IgG subclasses of immunoglobins containing kappa and lambda light chains with different affinities regulating their clearance and inhibiting the formation of insoluble immune complexes. Tethers plasminogen to the cell surface. Binds T-cells and alters the cell morphology. Modulates angiogenesis by blocking the CD6-mediated antiangiongenic effect of thrombospondins, THBS1 and THBS2 ().

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Product Notes

The HRG hrg (Catalog #AAA2111282) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Histidine Rich Glycoprotein (HRG) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Histidine Rich Glycoprotein (HRG) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). WB: 0.2-2ug/mL; 1:500-5000 IHC: 5-20ug/mL; 1:50-200 ICC: 5-20ug/mL; 1:50-200. Researchers should empirically determine the suitability of the HRG hrg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Leu19~Lys5 25. It is sometimes possible for the material contained within the vial of "Histidine Rich Glycoprotein (HRG), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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