Orexin (OX) Recombinant Protein | OX recombinant protein

Recombinant Orexin (OX)

Cat. Num. AAA2105259

• Gene Names: HCRT; OX; PPOX; NRCLP1
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Orexin (OX); Recombinant Orexin (OX); HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor; OX recombinant protein

• Host: Host: E Coli; Source: Prokaryotic Expression
• Purity/Purification: > 90%
• Form/Format: Freeze-Dried Powder; 20mM Tris, 150mM NaCl, pH8.0, Containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Sequence Positions: Gln34~Met97
• Sequence Length: 131

• Applicable Applications for OX recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Species: Human
• Tag: Two N-terminal-Tags, His-Tag and SUMO-Tag
• Isoelectric Point: 9.9
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Subcellular Location: Cytoplasm
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 4557635
• NCBI GeneID: 3060
• NCBI Accession #: NP_001515.1
• NCBI GenBank Nucleotide #: NM_001524.1
• UniProt Accession #: O43612
• Molecular Weight: Predicted MW: 20.5kDa; Accurate MW: 21kDa
• NCBI Official Full Name: orexin
• NCBI Official Synonym Full Names: hypocretin neuropeptide precursor
• NCBI Official Symbol: HCRT
• NCBI Official Synonym Symbols: OX; PPOX; NRCLP1
• NCBI Protein Information: orexin
• UniProt Protein Name: Orexin
• UniProt Gene Name: HCRT
• UniProt Synonym Gene Names: OX; PPORX; PPOX; Hcrt; Hcrt1; Hcrt2
• UniProt Entry Name: OREX_HUMAN

NCBI Description

This gene encodes a hypothalamic neuropeptide precursor protein that gives rise to two mature neuropeptides, orexin A and orexin B, by proteolytic processing. Orexin A and orexin B, which bind to orphan G-protein coupled receptors HCRTR1 and HCRTR2, function in the regulation of sleep and arousal. This neuropeptide arrangement may also play a role in feeding behavior, metabolism, and homeostasis. [provided by RefSeq, Jan 2010]

Uniprot Description

HCRT: Neuropeptides that play a significant role in the regulation of food intake and sleep-wakefulness, possibly by coordinating the complex behavioral and physiologic responses of these complementary homeostatic functions. A broader role in the homeostatic regulation of energy metabolism, autonomic function, hormonal balance and the regulation of body fluids, is also suggested. Orexin-A binds to both OX1R and OX2R with a high affinity, whereas orexin-B binds only to OX2R with a similar high affinity. Defects in HCRT are the cause of narcolepsy type 1 (NRCLP1). Narcolepsy is a neurological disabling sleep disorder, characterized by excessive daytime sleepiness, sleep fragmentation, symptoms of abnormal rapid-eye-movement (REM) sleep, such as cataplexy, hypnagogic hallucinations, and sleep paralysis. Cataplexy is a sudden loss of muscle tone triggered by emotions, which is the most valuable clinical feature used to diagnose narcolepsy. Human narcolepsy is primarily a sporadically occurring disorder but familial clustering has been observed. Human narcolepsy is associated with a deficient orexin system. Orexins are absent and/or greatly diminished in the brain and cerebrospinal fluid (CSF) of most narcoleptic patients. Belongs to the orexin family.

Protein type: Hormone

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: synaptic vesicle; rough endoplasmic reticulum; perinuclear region of cytoplasm; extracellular region; cell junction; secretory granule

Molecular Function: type 2 hypocretin receptor binding; type 1 hypocretin receptor binding

Biological Process: eating behavior; negative regulation of potassium ion transport; regulation of neurotransmitter secretion; synaptic transmission; elevation of cytosolic calcium ion concentration; negative regulation of transmission of nerve impulse; neuropeptide signaling pathway; negative regulation of DNA replication; protein kinase C activation; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); positive regulation of calcium ion transport; regulation of excitatory postsynaptic membrane potential; positive regulation of transmission of nerve impulse

Disease: Narcolepsy 1