Activity Data
(Figure. The binding activity of TK1 with GAPDH.Thymidine kinase 1 (TK1), is a human thymidine kinase. Thymidine kinase has been making a growing impact in the cancer research community. It has been found that elevated blood serum levels of TK-1 correlates with metastatic capabilities of the cancer and thereby can be used to detect malignant types of cancer, furthermore TK-1 has been found to show up in blood serum even before clinical symptoms even start to show. Besides, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) has been identified as an interactor of TK1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TK1 and recombinant human GAPDH. Briefly, TK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GAPDH-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TK1 and GAPDH was shown in Figure 1, and this effect was in a dose dependent manner.)
Thymidine Kinase 1, Soluble (TK1) Active Protein | TK1 active protein
Active Thymidine Kinase 1, Soluble (TK1)
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Activity Data
(Figure. The binding activity of TK1 with GAPDH.Thymidine kinase 1 (TK1), is a human thymidine kinase. Thymidine kinase has been making a growing impact in the cancer research community. It has been found that elevated blood serum levels of TK-1 correlates with metastatic capabilities of the cancer and thereby can be used to detect malignant types of cancer, furthermore TK-1 has been found to show up in blood serum even before clinical symptoms even start to show. Besides, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) has been identified as an interactor of TK1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TK1 and recombinant human GAPDH. Briefly, TK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GAPDH-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TK1 and GAPDH was shown in Figure 1, and this effect was in a dose dependent manner.)
Activity Data
(Figure. The binding activity of TK1 with GAPDH.Thymidine kinase 1 (TK1), is a human thymidine kinase. Thymidine kinase has been making a growing impact in the cancer research community. It has been found that elevated blood serum levels of TK-1 correlates with metastatic capabilities of the cancer and thereby can be used to detect malignant types of cancer, furthermore TK-1 has been found to show up in blood serum even before clinical symptoms even start to show. Besides, Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) has been identified as an interactor of TK1, thus a binding ELISA assay was conducted to detect the interaction of recombinant human TK1 and recombinant human GAPDH. Briefly, TK1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to GAPDH-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TK1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of TK1 and GAPDH was shown in Figure 1, and this effect was in a dose dependent manner.)
NCBI and Uniprot Product Information
Accurate MW: 55kDa
NCBI Description
The protein encoded by this gene is a cytosolic enzyme that catalyzes the addition of a gamma-phosphate group to thymidine. This creates dTMP and is the first step in the biosynthesis of dTTP, which is one component required for DNA replication. The encoded protein, whose levels fluctuate depending on the cell cycle stage, can act as a low activity dimer or a high activity tetramer. High levels of this protein have been used as a biomarker for diagnosing and categorizing many types of cancers. [provided by RefSeq, Oct 2016]
Uniprot Description
TK: cytosolic thymidine kinase. Phosphorylated during mitosis. Its enzymatic activity is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
Protein type: Nucleotide Metabolism - pyrimidine; EC 2.7.1.21; Xenobiotic Metabolism - drug metabolism - other enzymes; Kinase, other
Chromosomal Location of Human Ortholog: 17q23.2-q25.3
Cellular Component: cytosol
Molecular Function: identical protein binding; nucleoside kinase activity; zinc ion binding; thymidine kinase activity; ATP binding
Biological Process: response to nutrient levels; skeletal muscle cell proliferation; nucleobase, nucleoside and nucleotide metabolic process; response to toxin; liver development; fetal process involved in parturition; deoxyribonucleoside monophosphate biosynthetic process; protein homotetramerization; response to cortisol stimulus; pyrimidine base metabolic process; gut development; response to copper ion; nucleotide biosynthetic process; nucleobase, nucleoside, nucleotide and nucleic acid metabolic process; thymidine metabolic process; pyrimidine nucleoside salvage; phosphorylation
Research Articles on TK1
Similar Products
Product Notes
The TK1 tk1 (Catalog #AAA2105175) is an Active Protein and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Ser2~Asn234. AAA Biotech's Thymidine Kinase 1, Soluble (TK1) can be used in a range of immunoassay formats including, but not limited to, Cell Culture, Activity Assays. Researchers should empirically determine the suitability of the TK1 tk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thymidine Kinase 1, Soluble (TK1), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
