Human ATPase Family, AAA Domain Containing Protein 3A (ATAD3A) ELISA Kit | ATAD3A elisa kit

Human ATPase Family, AAA Domain Containing Protein 3A (ATAD3A) ELISA Kit

Cat. Num. AAA2105004

• Gene Names: ATAD3A; HAYOS
• Reactivity: Human
• Synonyms: ATPase Family; AAA Domain Containing Protein 3A (ATAD3A); Human ATPase Family; AAA Domain Containing Protein 3A (ATAD3A) ELISA Kit; ATAD3A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of AST. No significant cross-reactivity or interference between AST and analogues was observed.
• Sequence Length: 612

• Application Notes: Enzyme-linked immunosorbent assay for Antigen Detection.
• Samples: Rat Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 7.8-500ng/mL
• Sensitivity: 3.4ng/mL.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level AST were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level AST were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATAD3A elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AST in rat serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to AST. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to AST. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain AST, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of AST in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ATAD3A elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 119576592
• NCBI GeneID: 55210
• Molecular Weight: 66,218 Da
• NCBI Official Full Name: ATPase family, AAA domain containing 3A, isoform CRA_c
• NCBI Official Synonym Full Names: ATPase family, AAA domain containing 3A
• NCBI Official Symbol: ATAD3A
• NCBI Official Synonym Symbols: HAYOS
• NCBI Protein Information: ATPase family AAA domain-containing protein 3A
• UniProt Protein Name: ATPase family AAA domain-containing protein 3A
• Protein Family: ATPase family AAA domain-containing protein
• UniProt Gene Name: ATAD3A

NCBI Description

This gene encodes a ubiquitously expressed mitochondrial membrane protein that contributes to mitochondrial dynamics, nucleoid organization, protein translation, cell growth, and cholesterol metabolism. This gene is a member of the ATPase family AAA-domain containing 3 gene family which, in humans, includes two other paralogs. Naturally occurring mutations in this gene are associated with distinct neurological syndromes including Harel-Yoon syndrome. High-level expression of this gene is associated with poor survival in breast cancer patients. A homozygous knockout of the orthologous gene in mice results in embryonic lethality at day 7.5 due to growth retardation and defective development of the trophoblast lineage. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2017]

Uniprot Description

Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level. May play an important role in mitochondrial protein synthesis. May also participate in mitochondrial DNA replication. May bind to mitochondrial DNA D-loops and contribute to nucleoid stability. Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis.