Rabbit anti-Human CHN2 Polyclonal Antibody | anti-CHN2 antibody

CHN2, ID (CHN2, ARHGAP3, BCH, Beta-chimaerin, Beta-chimerin, Rho GTPase-activating protein 3) (PE)

Cat. Num. AAA6285732

• Gene Names: CHN2; BCH; CHN2-3; ARHGAP3; RHOGAP3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CHN2; Polyclonal Antibody; ID (CHN2; ARHGAP3; BCH; Beta-chimaerin; Beta-chimerin; Rho GTPase-activating protein 3) (PE); Rho GTPase-activating protein 3; anti-CHN2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-CHN2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: CHN2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 162~192 amino acids from the Central region of human CHN2.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of CHN2 Antibody (Center) in Hela cell line lysates (35ug/lane). CHN2 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of CHN2 Antibody (Center) in mouse liver tissue lysates (35ug/lane). CHN2 (arrow) was detected using the purified Pab.)

Related Product Information for anti-CHN2 antibody

This gene is a member of the chimerin family and encodes a protein with a phorbol-ester/DAG-type zinc finger, a Rho-GAP domain and an SH2 domain. This protein has GTPase-activating protein activity that is regulated by phospholipid binding and binding of diacylglycerol (DAG) induces translocation of the protein from the cytosol to the Golgi apparatus membrane. The protein plays a role in the proliferation and migration of smooth muscle cells. Decreased expression of this gene is associated with high-grade gliomas and breast tumors, and increased expression of this gene is associated with lymphomas. Mutations in this gene have been associated with schizophrenia in men. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Product Categories/Family for anti-CHN2 antibody

Antibodies; GTPase; Rab; Ras; Rho Proteins

References

Takefuji, M., et al. J. Hum. Genet. 55(1):42-49(2010). Suliman, S.G., et al. Diabetes 58(12):2954-2961(2009). Siliceo, M., et al. J. Biol. Chem. 284(17):11354-11363(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 119614325
• NCBI GeneID: 1124
• UniProt Accession #: P52757; Q2M203; Q75MM2; A4D1A2; B3VCF1; B3VCF2; B3VCF3; B3VCF7; B3VCG1; C9J7B0; E9PGE0; F8QPL9
• Molecular Weight: – Da
• NCBI Official Full Name: chimerin (chimaerin) 2
• NCBI Official Synonym Full Names: chimerin 2
• NCBI Official Symbol: CHN2
• NCBI Official Synonym Symbols: BCH; CHN2-3; ARHGAP3; RHOGAP3
• NCBI Protein Information: beta-chimaerin
• UniProt Protein Name: Beta-chimaerin
• Protein Family: Beta-chimaerin
• UniProt Gene Name: CHN2
• UniProt Synonym Gene Names: ARHGAP3; BCH

NCBI Description

This gene encodes a guanosine triphosphate (GTP)-metabolizing protein that contains a phorbol-ester/diacylglycerol (DAG)-type zinc finger, a Rho-GAP domain, and an SH2 domain. The encoded protein translocates from the cytosol to the Golgi apparatus membrane upon binding by diacylglycerol (DAG). Activity of this protein is important in cell proliferation and migration, and expression changes in this gene have been detected in cancers. A mutation in this gene has also been associated with schizophrenia in men. Alternative transcript splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

GTPase-activating protein for p21-rac. Insufficient expression of beta-2 chimaerin is expected to lead to higher Rac activity and could therefore play a role in the progression from low-grade to high-grade tumors.