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Testing Data (Sequence)

Delta Like 1 Homolog (dLK1) Recombinant Protein | dLK1 recombinant protein

Recombinant Delta Like 1 Homolog (dLK1)

Gene Names
DLK1; DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1
Applications
SDS-Page, Western Blot
Purity
> 90%
Synonyms
Delta Like 1 Homolog (dLK1); Recombinant Delta Like 1 Homolog (dLK1); FA1; PREF1; Pref-1; ZOG; pG2; Protein delta homolog 1; Fetal antigen 1; dLK1 recombinant protein
Ordering
For Research Use Only!
Host
E.coli
Purity/Purification
> 90%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Concentration
20ug/mL (varies by lot)
Applicable Applications for dLK1 recombinant protein
Positive Control; Immunogen; SDS-PAGE (SDS); Western Blot (WB)
Application Notes
(May be suitable for use in other assays to be determined by the end user.)
Organism Species
Homo sapiens (Human)
Source
Prokaryotic expression
Residues
Ser206~Pro366
Tags
N-terminal His and GST Tag
Subcellular Location
Membrane, Cytoplasm
Traits
Freeze-dried powder
Usage
Reconstitute in ddH2O to a concentration of 0-0.5 mg/mL. Do not vortex.
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Testing Data

(Sequence)

Testing Data (Sequence)

Testing Data

(Figure . Gene Sequencing (extract) Figure. SDS-PAGE)

Testing Data (Figure . Gene Sequencing (extract) Figure. SDS-PAGE)

SDS-PAGE (SDS)

(Figure. SDS-PAGE)

SDS-PAGE (SDS) (Figure. SDS-PAGE)
Product Categories/Family for dLK1 recombinant protein

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted isoelectric point: 9.4
Predicted Molecular Mass: 47.8kDa
Accurate Molecular Mass: 48kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
protein delta homolog 1 isoform 2 preproprotein
NCBI Official Synonym Full Names
delta like non-canonical Notch ligand 1
NCBI Official Symbol
DLK1
NCBI Official Synonym Symbols
DLK; FA1; ZOG; pG2; DLK-1; PREF1; Delta1; Pref-1
NCBI Protein Information
protein delta homolog 1
UniProt Protein Name
Protein delta homolog 1
Protein Family
UniProt Gene Name
DLK1
UniProt Synonym Gene Names
DLK; DLK-1; FA1

NCBI Description

This gene encodes a transmembrane protein that contains multiple epidermal growth factor repeats that functions as a regulator of cell growth. The encoded protein is involved in the differentiation of several cell types including adipocytes. This gene is located in a region of chromosome 14 frequently showing unparental disomy, and is imprinted and expressed from the paternal allele. A single nucleotide variant in this gene is associated with child and adolescent obesity and shows polar overdominance, where heterozygotes carrying an active paternal allele express the phenotype, while mutant homozygotes are normal. [provided by RefSeq, Nov 2015]

Uniprot Description

DLK1: May have a role in neuroendocrine differentiation. Monomer. Found within the stromal cells in close contact to the vascular structure of placental villi, yolk sac, fetal liver, adrenal cortex and pancreas and in the beta cells of the islets of Langerhans in the adult pancreas. Found also in some forms of neuroendocrine lung tumor tissue. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 14q32.2

Cellular Component: extracellular space; integral component of membrane

Molecular Function: calcium ion binding

Biological Process: cell differentiation; negative regulation of Notch signaling pathway

Research Articles on dLK1

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Product Notes

The dLK1 dlk1 (Catalog #AAA2097707) is a Recombinant Protein produced from E.coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Delta Like 1 Homolog (dLK1) can be used in a range of immunoassay formats including, but not limited to, Positive Control; Immunogen; SDS-PAGE (SDS); Western Blot (WB). (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the dLK1 dlk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Delta Like 1 Homolog (dLK1), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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