Apolipoprotein B100 (APOB100) Recombinant Protein | APOB100 recombinant protein

Recombinant Apolipoprotein B100 (APOB100)

Cat. Num. AAA2097662

• Gene Names: APOB; FLDB; LDLCQ4; apoB-48; apoB-100
• Reactivity: Homo Sapiens (Human)
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Apolipoprotein B100 (APOB100); Recombinant Apolipoprotein B100 (APOB100); Apo-B100; APOB100 recombinant protein

• Host: E.coli
• Reactivity: Homo Sapiens (Human)
• Purity/Purification: > 90%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
• Concentration: Original Concentration: 450ug/mL (varies by lot)

• Applicable Applications for APOB100 recombinant protein: Positive Control; Immunogen; SDS-PAGE; Western Blot (WB).
• Application Notes: (May be suitable for use in other assays to be determined by the end user.)
• Residues: His3365~Glu3548
• Tags: N-terminal His Tag
• Subcellular Location: Secreted, Cytoplasm, Chromosome
• Traits: Freeze-dried powder
• Predicted isoelectric point: 9.5
• Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:; 1. Splice variants: Alternative splicing may create different sized proteins from the same gene.; 2. Relative charge: The composition of amino acids may affects the charge of the protein.; 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.; 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.; 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Usage: Reconstitute in 20mM Tris, 150mM NaCL (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 2-8 degree C for one month. ; Aliquot and store at -80 degree C for 12 months.; ; Stability Test:; The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Sequence

Gene Sequencing (Extract)

SDS-PAGE

Product Categories/Family for APOB100 recombinant protein

Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 105990532
• NCBI GeneID: 338
• NCBI Accession #: NP_000375.2
• NCBI GenBank Nucleotide #: NM_000384.2
• UniProt Accession #: P04114; O00502; P78479; P78480; P78481; Q13779; Q13785; Q13786; Q13787; Q13788; Q4ZG63; Q53QC8
• Molecular Weight: Predicted Molecular Mass: 21.4kDa; Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions.
• NCBI Official Full Name: apolipoprotein B-100
• NCBI Official Synonym Full Names: apolipoprotein B
• NCBI Official Symbol: APOB
• NCBI Official Synonym Symbols: FLDB; LDLCQ4; apoB-48; apoB-100
• NCBI Protein Information: apolipoprotein B-100
• UniProt Protein Name: Apolipoprotein B-100
• UniProt Gene Name: APOB
• UniProt Synonym Gene Names: Apo B-100; Apo B-48

NCBI Description

This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Jul 2008]

Uniprot Description

APOB: Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. Defects in APOB are a cause of familial hypobetalipoproteinemia type 1 (FHBL1). A disorder characterized by highly reduced plasma concentrations of low density lipoproteins, and dietary fat malabsorption. Clinical presentation may vary from no symptoms to severe gastrointestinal and neurological dysfunction similar to abetalipoproteinemia. Defects in APOB are a cause of familial ligand-defective apolipoprotein B-100 (FDB). FDB is a dominantly inherited disorder of lipoprotein metabolism leading to hypercholesterolemia and increased proneness to coronary artery disease (CAD). The plasma cholesterol levels are dramatically elevated due to impaired clearance of LDL particles by defective APOB/E receptors. Defects in APOB associated with defects in other genes (polygenic) can contribute to hypocholesterolemia.

Protein type: Carrier; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: cell soma; chylomicron; chylomicron remnant; cytoplasm; cytosol; early endosome; endocytic vesicle lumen; endoplasmic reticulum exit site; endoplasmic reticulum lumen; endoplasmic reticulum membrane; endosome membrane; extracellular exosome; extracellular region; extracellular space; intermediate-density lipoprotein particle; intracellular membrane-bound organelle; low-density lipoprotein particle; lysosomal lumen; mature chylomicron; plasma membrane; smooth endoplasmic reticulum; very-low-density lipoprotein particle

Molecular Function: cholesterol transporter activity; heparin binding; low-density lipoprotein receptor binding; phospholipid binding; protein binding

Biological Process: artery morphogenesis; cellular protein metabolic process; cellular response to prostaglandin stimulus; cellular response to tumor necrosis factor; cholesterol efflux; cholesterol homeostasis; cholesterol metabolic process; cholesterol transport; chylomicron assembly; chylomicron remnant clearance; chylomicron remodeling; fertilization; in utero embryonic development; leukocyte migration; lipoprotein biosynthetic process; lipoprotein catabolic process; lipoprotein transport; low-density lipoprotein particle clearance; low-density lipoprotein particle remodeling; nervous system development; positive regulation of cholesterol storage; positive regulation of gene expression; positive regulation of lipid storage; positive regulation of macrophage derived foam cell differentiation; post-embryonic development; post-translational protein modification; receptor-mediated endocytosis; regulation of cholesterol biosynthetic process; response to carbohydrate stimulus; response to estradiol; response to lipopolysaccharide; response to selenium ion; response to virus; retinoid metabolic process; sperm motility; spermatogenesis; toll-like receptor signaling pathway; triacylglycerol mobilization; triglyceride catabolic process; very-low-density lipoprotein particle assembly

Disease: Hypercholesterolemia, Autosomal Dominant, Type B; Hypobetalipoproteinemia, Familial, 1