Mouse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) ELISA Kit | RANTES elisa kit

Mouse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Mini Samples ELISA Kit

Cat. Num. AAA2089503

• Gene Names: Ccl5; SISd; Scya5; RANTES; TCP228; MuRantes
• Reactivity: Mouse
• Synonyms: Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES); Mouse Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) Mini Samples ELISA Kit; CCL5; SCYA5; SISd; EoCP; Chemokine C-C-Motif Ligand 5; Eosinophil Chemotactic Cytokine; T-cell Specific Protein p288; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 5; RANTES elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES).; No significant cross-reactivity or interference between Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) and analogues was observed.
• Sequence Length: 91

• Assay Type: Double-antibody Sandwich
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Related Product Information for RANTES elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Regulated On Activation In Normal T-Cell Expressed And Secreted (RANTES) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for RANTES elisa kit

Cytokine; Tumor immunity; Infection immunity; Immune molecule

NCBI and Uniprot Product Information

• NCBI GI #: 164698428
• NCBI GeneID: 20304
• NCBI Accession #: NP_038681.2
• NCBI GenBank Nucleotide #: NM_013653.3
• UniProt Accession #: P30882
• Molecular Weight: 10,071 Da
• NCBI Official Full Name: C-C motif chemokine 5
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 5
• NCBI Official Symbol: Ccl5
• NCBI Official Synonym Symbols: SISd; Scya5; RANTES; TCP228; MuRantes
• NCBI Protein Information: C-C motif chemokine 5
• UniProt Protein Name: C-C motif chemokine 5
• UniProt Gene Name: Ccl5
• UniProt Synonym Gene Names: Scya5

Uniprot Description

CCL5: Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T- cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. By mitogens. T-cell and macrophage specific. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Cell adhesion; Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11 C|11 50.66 cM

Cellular Component: cytoplasm; extracellular space

Molecular Function: CCR1 chemokine receptor binding; CCR5 chemokine receptor binding; chemoattractant activity; chemokine activity; chemokine receptor antagonist activity; chemokine receptor binding; heparin binding; phosphoinositide phospholipase C activity; phospholipase activator activity; protein homodimerization activity; protein kinase activity; protein self-association; receptor signaling protein tyrosine kinase activator activity

Biological Process: calcium ion transport; cell-cell signaling; cellular calcium ion homeostasis; cellular protein complex assembly; eosinophil chemotaxis; exocytosis; G-protein coupled receptor protein signaling pathway; inflammatory response; leukocyte adhesion; lipopolysaccharide-mediated signaling pathway; lymphocyte chemotaxis; MAPKKK cascade; negative regulation of G-protein coupled receptor protein signaling pathway; negative regulation of viral genome replication; neutrophil activation; neutrophil chemotaxis; phospholipase D activation; positive regulation of angiogenesis; positive regulation of calcium ion transport; positive regulation of cell adhesion; positive regulation of cell migration; positive regulation of cell-cell adhesion mediated by integrin; positive regulation of cellular biosynthetic process; positive regulation of defense response to virus by host; positive regulation of epithelial cell proliferation; positive regulation of fever; positive regulation of GTPase activity; positive regulation of homotypic cell-cell adhesion; positive regulation of inflammatory response; positive regulation of neuron differentiation; positive regulation of osteoclast differentiation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of phosphorylation; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of T cell proliferation; positive regulation of tyrosine phosphorylation of STAT protein; protein kinase B signaling cascade; protein tetramerization; pseudopodium formation; regulation of insulin secretion; regulation of T cell activation; response to cytokine stimulus; response to toxin