Loading...

Skip to main content

Call us on + 1 (800) 604-9114 for more information about our products

Looking for specific datasheet Manual/COA/MSDS?
Request a Manual/COA/MSDS

Interested to get a quote about our products?
Request a Quote

Mouse Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit | GCSF elisa kit

Mouse Colony Stimulating Factor 3, Granulocyte (GCSF) Mini Samples ELISA Kit

Gene Names
Csf3; Csfg; G-CSF; MGI-IG
Reactivity
Mouse
Synonyms
Colony Stimulating Factor 3; Granulocyte (GCSF); Mouse Colony Stimulating Factor 3; Granulocyte (GCSF) Mini Samples ELISA Kit; CSF3; G-CSF; Granulocyte Colony Stimulating Factor; Pluripoietin; Filgrastim; Lenograstim; GCSF elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF).
No significant cross-reactivity or interference between Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF) and analogues was observed.
Sequence Length
208
Assay Type
Double-antibody Sandwich
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Related Product Information for GCSF elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mini Samples Colony Stimulating Factor 3, Granulocyte (GCSF) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for GCSF elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
22,421 Da
NCBI Official Full Name
granulocyte colony-stimulating factor
NCBI Official Synonym Full Names
colony stimulating factor 3 (granulocyte)
NCBI Official Symbol
Csf3
NCBI Official Synonym Symbols
Csfg; G-CSF; MGI-IG
NCBI Protein Information
granulocyte colony-stimulating factor
UniProt Protein Name
Granulocyte colony-stimulating factor
UniProt Gene Name
Csf3
UniProt Synonym Gene Names
Csfg; G-CSF

Uniprot Description

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11 D|11 62.45 cM

Cellular Component: extracellular space

Molecular Function: cytokine activity; enzyme binding; granulocyte colony-stimulating factor receptor binding; growth factor activity

Biological Process: positive regulation of actin filament polymerization; positive regulation of cell proliferation; positive regulation of myeloid cell differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein binding; positive regulation of protein kinase B signaling cascade; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter

Research Articles on GCSF

Similar Products

Product Notes

The Mouse GCSF csf3 (Catalog #AAA2089481) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2089481 ELISA Kit recognizes Mouse GCSF. It is sometimes possible for the material contained within the vial of "Colony Stimulating Factor 3, Granulocyte (GCSF), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

Item has been added to Shopping Cart

If you are ready to order, navigate to Shopping Cart and get ready to checkout.

Looking for a specific manual?
Request a Manual

Request more Information

Please complete the form below and a representative will contact you as soon as possible.

Request a Manual

Please complete the form below and a representative will contact you as soon as possible.

Request a Quote

Please complete the form below and a representative will contact you as soon as possible.