Human Stanniocalcin 2 (STC2) DIY ELISA Kit | STC2 diy elisa kit

Human Stanniocalcin 2 (STC2) ELISA Kit DIY Materials

Cat. Num. AAA2089372

• Gene Names: STC2; STC-2; STCRP
• Reactivity: Human
• Synonyms: Stanniocalcin 2 (STC2); Human Stanniocalcin 2 (STC2) ELISA Kit DIY Materials; STCRP; Stanniocalcin-related protein; STC2 diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Stanniocalcin 2 (STC2). No significant cross-reactivity or interference between Stanniocalcin 2 (STC2) and analogues was observed.
• Sequence Length: 302

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 31.25-2000pg/mL
• Sensitivity: 11.2pg/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for STC2 diy elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 15530321
• NCBI GeneID: 8614
• Molecular Weight: 33,249 Da
• NCBI Official Full Name: Stanniocalcin 2
• NCBI Official Synonym Full Names: stanniocalcin 2
• NCBI Official Symbol: STC2
• NCBI Official Synonym Symbols: STC-2; STCRP
• NCBI Protein Information: stanniocalcin-2
• UniProt Protein Name: Stanniocalcin-2
• Protein Family: Stanniocalcin
• UniProt Gene Name: STC2
• UniProt Synonym Gene Names: STC-2; STC-related protein; STCRP

NCBI Description

This gene encodes a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression of this gene is induced by estrogen and altered in some breast cancers. [provided by RefSeq, Jul 2008]

Uniprot Description

STC2: a secreted protein that has an anti-hypocalcemic action on calcium and phosphate homeostasis. Expressed in a variety of tissues including muscle, heart, pancreas, kidney, spleen, prostate, small intestine, colon and peripheral blood leucocytes.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: endoplasmic reticulum; extracellular space; perinuclear region of cytoplasm

Molecular Function: enzyme binding; heme binding; protein homodimerization activity

Biological Process: regulation of hormone biosynthetic process