Mouse Obestatin (OB) DIY ELISA Kit | OB diy elisa kit

Mouse Obestatin (OB) ELISA Kit DIY Materials

Cat. Num. AAA2089242

• Gene Names: LEP; OB; OBS; LEPD
• Reactivity: Mouse
• Synonyms: Obestatin (OB); Mouse Obestatin (OB) ELISA Kit DIY Materials; OB diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Obestatin (OB). No significant cross-reactivity or interference between Obestatin (OB) and analogues was observed.
• Sequence Length: 167

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Competitive Inhibition ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 61.7-5,000pg/mL
• Sensitivity: 27.5pg/mL
• Reagent Contents: Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

NCBI and Uniprot Product Information

• NCBI GI #: 623332
• NCBI GeneID: 3952
• Molecular Weight: 18,641 Da
• NCBI Official Full Name: ob
• NCBI Official Synonym Full Names: leptin
• NCBI Official Symbol: LEP
• NCBI Official Synonym Symbols: OB; OBS; LEPD
• NCBI Protein Information: leptin
• UniProt Protein Name: Leptin
• UniProt Gene Name: LEP

NCBI Description

This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008]

Uniprot Description

leptin: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in LEP may be a cause of obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Belongs to the leptin family.

Protein type: Cell development/differentiation; Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7q32.1

Cellular Component: extracellular region; extracellular space

Molecular Function: hormone activity; peptide hormone receptor binding

Biological Process: adult feeding behavior; angiogenesis; intestinal absorption; leptin-mediated signaling pathway; lipid metabolic process; negative regulation of appetite; negative regulation of autophagy; negative regulation of glucose import; placenta development; positive regulation of developmental growth; positive regulation of JAK-STAT cascade; positive regulation of MAPKKK cascade; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of T cell proliferation; positive regulation of TOR signaling pathway; prostaglandin secretion; regulation of angiogenesis; regulation of bone remodeling; regulation of cell cycle; regulation of endothelial cell proliferation; regulation of natural killer cell activation; regulation of natural killer cell mediated cytotoxicity; regulation of natural killer cell proliferation; regulation of nitric-oxide synthase activity; response to insulin stimulus; sexual reproduction; T cell differentiation; tyrosine phosphorylation of STAT protein

Disease: Leptin Deficiency