Human Vitamin D Binding Protein (DBP) DIY ELISA Kit | DBP diy elisa kit

Human Vitamin D Binding Protein (DBP) ELISA Kit DIY Materials

Cat. Num. AAA2089162

• Gene Names: GC; DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
• Reactivity: Human
• Synonyms: Vitamin D Binding Protein (DBP); Human Vitamin D Binding Protein (DBP) ELISA Kit DIY Materials; GC; VDBG; VDBP; Group-Specific Component; Gc-globulin; DBP diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Vitamin D Binding Protein (DBP). No significant cross-reactivity or interference between Vitamin D Binding Protein (DBP) and analogues was observed.
• Sequence Length: 493

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma and other biological fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.074ng/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for DBP diy elisa kit

Metabolic pathway

NCBI and Uniprot Product Information

• NCBI GI #: 324021745
• NCBI GeneID: 2638
• NCBI Accession #: NP_001191236.1
• NCBI GenBank Nucleotide #: NM_001204307.1
• UniProt Accession #: P02774; Q16309; Q16310; Q53F31; Q6GTG1; B4DPP2; D6RAK8
• Molecular Weight: 55,123 Da
• NCBI Official Full Name: vitamin D-binding protein isoform 3
• NCBI Official Synonym Full Names: GC, vitamin D binding protein
• NCBI Official Symbol: GC
• NCBI Official Synonym Symbols: DBP; GRD3; VDBG; VDBP; GcMAF; DBP/GC; Gc-MAF; HEL-S-51
• NCBI Protein Information: vitamin D-binding protein
• UniProt Protein Name: Vitamin D-binding protein
• Protein Family: Remorin
• UniProt Gene Name: GC
• UniProt Synonym Gene Names: DBP-maf

NCBI Description

The protein encoded by this gene belongs to the albumin gene family. It is a multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid and on the surface of many cell types. It binds to vitamin D and its plasma metabolites and transports them to target tissues. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Feb 2011]

Uniprot Description

GC: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes. Belongs to the ALB/AFP/VDB family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cytosol; extracellular region; extracellular space; lysosomal lumen

Molecular Function: actin binding; vitamin D binding

Biological Process: vitamin D metabolic process

Disease: Graves Disease, Susceptibility To, 1