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Human Thyrotropin Releasing Hormone (TRH) DIY ELISA Kit | TRH diy elisa kit

Human Thyrotropin Releasing Hormone (TRH) ELISA Kit DIY Materials

Gene Names
TRH; TRF; Pro-TRH
Reactivity
Human
Synonyms
Thyrotropin Releasing Hormone (TRH); Human Thyrotropin Releasing Hormone (TRH) ELISA Kit DIY Materials; TRF; Thyrotropin-Releasing Factor; Thyroliberin; Protirelin; Pro-tirelin; Prothyroliberin; TSH-releasing factor; TRH diy elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Thyrotropin Releasing Hormone (TRH). No significant cross-reactivity or interference between Thyrotropin Releasing Hormone (TRH) and analogues was observed.
Sequence Length
242
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Competitive Inhibition ELISA for Antigen Detection
Samples
Serum, plasma and other biological fluids.
Detection Range
6.17-500pg/mL
Sensitivity
2.63pg/mL
Reagent Contents
Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for TRH diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,404 Da
NCBI Official Full Name
thyrotropin releasing hormone preproprotein
NCBI Official Synonym Full Names
thyrotropin releasing hormone
NCBI Official Symbol
TRH
NCBI Official Synonym Symbols
TRF; Pro-TRH
NCBI Protein Information
thyrotropin releasing hormone
UniProt Protein Name
Pro-thyrotropin-releasing hormone
UniProt Gene Name
TRH
UniProt Synonym Gene Names
Pro-TRH; TRH; TRF

NCBI Description

This gene encodes a member of the thyrotropin-releasing hormone family. Cleavage of the encoded proprotein releases mature thyrotropin-releasing hormone, which is a tripeptide hypothalamic regulatory hormone. The human proprotein contains six thyrotropin-releasing hormone tripeptides. Thyrotropin-releasing hormone is involved in the regulation and release of thyroid-stimulating hormone, as well as prolactin. Deficiency of this hormone has been associated with hypothalamic hypothyroidism. [provided by RefSeq, May 2013]

Uniprot Description

TRH: Functions as a regulator of the biosynthesis of TSH in the anterior pituitary gland and as a neurotransmitter/ neuromodulator in the central and peripheral nervous systems. May promote hair shaft elongation, prolonge the hair cycle growth phase (anagen) and antagonized its termination by TGFB2. May also increase proliferation and inhibited apoptosis of hair matrix keratinocytes. Belongs to the TRH family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: extracellular region; nucleus; plasma membrane; secretory granule

Biological Process: cell-cell signaling; eating behavior; histamine metabolic process; negative regulation of glutamate secretion; positive regulation of gamma-aminobutyric acid secretion; positive regulation of insulin secretion; signal transduction

Disease: Thyrotropin-releasing Hormone Deficiency

Research Articles on TRH

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Product Notes

The Human TRH trh (Catalog #AAA2088862) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088862 DIY ELISA Kit recognizes Human TRH. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the TRH trh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thyrotropin Releasing Hormone (TRH), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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