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Rat Calcitonin (CT) DIY ELISA Kit | CT diy elisa kit

Rat Calcitonin (CT) ELISA Kit DIY Materials

Gene Names
CALCA; CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
Reactivity
Rat
Synonyms
Calcitonin (CT); Rat Calcitonin (CT) ELISA Kit DIY Materials; CALCA; CALC1; CGRP-I; CGRP1; KC; CCP; PDN-21; Katacalcin; Calcitonin-Related Polypeptide Alpha; Calcitonin carboxyl-terminal peptide; CT diy elisa kit
Ordering
For Research Use Only!
Reactivity
Rat
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Calcitonin (CT). No significant cross-reactivity or interference between Calcitonin (CT) and analogues was observed.
Sequence Length
141
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Competitive Inhibition ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
12.35-1000pg/mL
Sensitivity
4.95pg/mL
Reagent Contents
Capture Antibody, Biontin-labeled Competitor, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 2. (Catalog MBS2089472)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for CT diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
796
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
13,898 Da
NCBI Official Full Name
calcitonin isoform CT preproprotein
NCBI Official Synonym Full Names
calcitonin related polypeptide alpha
NCBI Official Symbol
CALCA
NCBI Official Synonym Symbols
CT; KC; PCT; CGRP; CALC1; CGRP1; CGRP-I
NCBI Protein Information
calcitonin; calcitonin gene-related peptide 1
UniProt Protein Name
Calcitonin
UniProt Gene Name
CALCA
UniProt Synonym Gene Names
CALC1; CCP

NCBI Description

This gene encodes the peptide hormones calcitonin, calcitonin gene-related peptide and katacalcin by tissue-specific alternative RNA splicing of the gene transcripts and cleavage of inactive precursor proteins. Calcitonin is involved in calcium regulation and acts to regulate phosphorus metabolism. Calcitonin gene-related peptide functions as a vasodilator and as an antimicrobial peptide while katacalcin is a calcium-lowering peptide. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Aug 2014]

Uniprot Description

CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11p15.2

Cellular Component: extracellular region; extracellular space

Molecular Function: calcitonin receptor binding; identical protein binding; protein binding

Biological Process: activation of protein kinase activity; cellular protein metabolic process; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); embryo implantation; monocyte chemotaxis; negative regulation of bone resorption; negative regulation of transcription, DNA-dependent; positive regulation of adenylate cyclase activity

Research Articles on CT

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Product Notes

The Rat CT calca (Catalog #AAA2088591) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088591 DIY ELISA Kit recognizes Rat CT. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the CT calca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calcitonin (CT), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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