Human Neurotrophin 3 (NT3) DIY ELISA Kit | NT3 diy elisa kit

Human Neurotrophin 3 (NT3) ELISA Kit DIY Materials

Cat. Num. AAA2088414

• Gene Names: NTF3; NT3; HDNF; NGF2; NT-3; NGF-2
• Reactivity: Human
• Synonyms: Neurotrophin 3 (NT3); Human Neurotrophin 3 (NT3) ELISA Kit DIY Materials; NTF3; HDNF; NGF2; Nerve Growth Factor 2; Neurotrophic Factor; NT3 diy elisa kit

• Reactivity: Human
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Neurotrophin 3 (NT3). No significant cross-reactivity or interference between Neurotrophin 3 (NT3) and analogues was observed.
• Sequence Length: 257

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
• Detection Range: 78.12-5000pg/mL
• Sensitivity: 33pg/mL.
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for NT3 diy elisa kit

Cytokine; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 4505469
• NCBI GeneID: 4908
• NCBI Accession #: NP_002518.1
• NCBI GenBank Nucleotide #: NM_002527.4
• UniProt Accession #: P20783; Q6FH50; B7Z1T5
• Molecular Weight: 30,800 Da
• NCBI Official Full Name: neurotrophin-3 isoform 2 preproprotein
• NCBI Official Synonym Full Names: neurotrophin 3
• NCBI Official Symbol: NTF3
• NCBI Official Synonym Symbols: NT3; HDNF; NGF2; NT-3; NGF-2
• NCBI Protein Information: neurotrophin-3
• UniProt Protein Name: Neurotrophin-3
• UniProt Gene Name: NTF3
• UniProt Synonym Gene Names: NT-3; NGF-2

NCBI Description

The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq, Jul 2008]

Uniprot Description

NT-3: Seems to promote the survival of visceral and proprioceptive sensory neurons. Belongs to the NGF-beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: extracellular region

Molecular Function: chemoattractant activity; growth factor activity; neurotrophin p75 receptor binding; protein binding; receptor binding

Biological Process: activation of MAPK activity; activation of protein kinase B; cell-cell signaling; induction of positive chemotaxis; negative regulation of neuron apoptosis; negative regulation of peptidyl-tyrosine phosphorylation; nervous system development; neurite morphogenesis; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of receptor internalization; regulation of apoptosis; regulation of neuron differentiation; signal transduction; transmembrane receptor protein tyrosine kinase signaling pathway