Mouse Macrophage Derived Chemokine (MDC) DIY ELISA Kit | MDC diy elisa kit

Mouse Macrophage Derived Chemokine (MDC) ELISA Kit DIY Materials

Cat. Num. AAA2088406

• Gene Names: CCL22; MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• Reactivity: Mouse
• Synonyms: Macrophage Derived Chemokine (MDC); Mouse Macrophage Derived Chemokine (MDC) ELISA Kit DIY Materials; CCL22; ABCD1; DC/B-CK; SCYA22; STCP1; Chemokine C-C-Motif Ligand 22; Small Inducible Cytokine Subfamily A(Cys-Cys)Member 22; Stimulated T-Cell Chemotactic Protein 1; MDC diy elisa kit

• Reactivity: Mouse
• Specificity: The Abs in the kit have high sensitivity and excellent specificity for detection of Macrophage Derived Chemokine (MDC). No significant cross-reactivity or interference between Macrophage Derived Chemokine (MDC) and analogues was observed.
• Sequence Length: 93

• Application Notes: Main materials for "Do It Yourself ELISA Kit"
• Assay Type: Double-antibody Sandwich ELISA for Antigen Detection
• Samples: Serum, plasma and other biological fluids.
• Detection Range: 15.62-1000pg/mL
• Sensitivity: 5.8pg/mL
• Reagent Contents: Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
• Product Usage: 1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.; 2. Aspirate and wash 1 time.; 3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.; 4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
• Other Reagents Required: ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
• Preparation and Storage: Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.

Product Categories/Family for MDC diy elisa kit

Cytokine; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 1931581
• NCBI GeneID: 6367
• Molecular Weight: 10,625 Da
• NCBI Official Full Name: macrophage-derived chemokine
• NCBI Official Synonym Full Names: C-C motif chemokine ligand 22
• NCBI Official Symbol: CCL22
• NCBI Official Synonym Symbols: MDC; ABCD-1; SCYA22; STCP-1; DC/B-CK; A-152E5.1
• NCBI Protein Information: C-C motif chemokine 22
• UniProt Protein Name: C-C motif chemokine 22
• UniProt Gene Name: CCL22
• UniProt Synonym Gene Names: MDC; SCYA22

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q arm of chromosome 16. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, dendritic cells, natural killer cells and for chronically activated T lymphocytes. It also displays a mild activity for primary activated T lymphocytes and has no chemoattractant activity for neutrophils, eosinophils and resting T lymphocytes. The product of this gene binds to chemokine receptor CCR4. This chemokine may play a role in the trafficking of activated T lymphocytes to inflammatory sites and other aspects of activated T lymphocyte physiology. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL22: May play a role in the trafficking of activated/effector T-lymphocytes to inflammatory sites and other aspects of activated T-lymphocyte physiology. Chemotactic for monocytes, dendritic cells and natural killer cells. Mild chemoattractant for primary activated T-lymphocytes and a potent chemoattractant for chronically activated T-lymphocytes but has no chemoattractant activity for neutrophils, eosinophils, and resting T-lymphocytes. Binds to CCR4. Processed forms MDC(3-69), MDC(5-69) and MDC(7-69) seem not be active. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: extracellular region

Molecular Function: CCR chemokine receptor binding

Biological Process: cell-cell signaling; chemotaxis; G-protein coupled receptor protein signaling pathway; immune response; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of GTPase activity; response to virus; signal transduction