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Human Amphiregulin (AREG) DIY ELISA Kit | AREG diy elisa kit

Human Amphiregulin (AREG) ELISA Kit DIY Materials

Gene Names
AREG; AR; SDGF; AREGB; CRDGF
Reactivity
Human
Synonyms
Amphiregulin (AREG); Human Amphiregulin (AREG) ELISA Kit DIY Materials; AR; CRDGF; SDGF; Colorectum Cell-Derived Growth Factor; Schwannoma-Derived Growth Factor; AREG diy elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Amphiregulin (AREG). No significant cross-reactivity or interference between Amphiregulin (AREG) and analogues was observed.
Sequence Length
252
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
15.6-1,000pg/mL
Sensitivity
6.5pg/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for AREG diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
374
Molecular Weight
27,895 Da
NCBI Official Full Name
amphiregulin
NCBI Official Synonym Full Names
amphiregulin
NCBI Official Symbol
AREG
NCBI Official Synonym Symbols
AR; SDGF; AREGB; CRDGF
NCBI Protein Information
amphiregulin
UniProt Protein Name
Amphiregulin
Protein Family
UniProt Gene Name
AREG
UniProt Synonym Gene Names
CRDGF

NCBI Description

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]

Uniprot Description

AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: cell surface; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; extracellular space

Molecular Function: epidermal growth factor receptor binding; growth factor activity; protein binding

Biological Process: cell proliferation; cell-cell signaling; COPII coating of Golgi vesicle; epidermal growth factor receptor signaling pathway; ER to Golgi vesicle-mediated transport; G-protein coupled receptor protein signaling pathway; positive regulation of cell proliferation; positive regulation of DNA replication

Research Articles on AREG

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Product Notes

The Human AREG areg (Catalog #AAA2088294) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088294 DIY ELISA Kit recognizes Human AREG. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the AREG areg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Amphiregulin (AREG), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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