Human ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) ELISA Kit | ATP6AP2 elisa kit

Human ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) ELISA Kit

Cat. Num. AAA2088191

• Gene Names: ATP6AP2; PRR; M8-9; MRXE; RENR; XMRE; XPDS; HT028; MRXSH; ELDF10; ATP6IP2; MSTP009; APT6M8-9; ATP6M8-9
• Reactivity: Human
• Synonyms: ATPase; H+ Transporting; Lysosomal Accessory Protein 2 (ATP6AP2); Human ATPase; Lysosomal Accessory Protein 2 (ATP6AP2) ELISA Kit; ATP6IP2; CAPER; ELDF10; XMRE; Renin/prorenin receptor; Embryonic liver differentiation factor 10; Vacuolar ATP synthase membrane sector-associated protein M8-9; ATP6AP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2).; No significant cross-reactivity or interference between ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) and analogues was observed.
• Sequence Length: 350

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.115ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATP6AP2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for ATP6AP2 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 15011918
• NCBI GeneID: 10159
• NCBI Accession #: NP_005756.2
• NCBI GenBank Nucleotide #: NM_005765.2
• UniProt Accession #: O75787; Q5QTQ7; Q6T7F5; Q8NBP3; Q8NG15; Q96FV6; Q96LB5; Q9H2P8; Q9UG89; B7Z9I3
• Molecular Weight: 35,612 Da
• NCBI Official Full Name: renin receptor
• NCBI Official Synonym Full Names: ATPase H+ transporting accessory protein 2
• NCBI Official Symbol: ATP6AP2
• NCBI Official Synonym Symbols: PRR; M8-9; MRXE; RENR; XMRE; XPDS; HT028; MRXSH; ELDF10; ATP6IP2; MSTP009; APT6M8-9; ATP6M8-9
• NCBI Protein Information: renin receptor
• UniProt Protein Name: Renin receptor
• Protein Family: Renin receptor
• UniProt Gene Name: ATP6AP2
• UniProt Synonym Gene Names: ATP6IP2; CAPER; ELDF10; ATP6M8-9; V-ATPase M8.9 subunit

NCBI Description

This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6AP2: Functions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS). Defects in ATP6AP2 are a cause of mental retardation X- linked with epilepsy (MRXE). MRXE is a syndromic mental retardation. Patients manifest mild to moderate mental retardation associated with epilepsy, delays in motor milestones and speech acquisition in infancy.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: external side of plasma membrane; plasma membrane

Molecular Function: protein binding

Biological Process: angiotensin maturation; eye pigmentation; positive regulation of transforming growth factor-beta1 production; positive regulation of Wnt receptor signaling pathway; regulation of MAPKKK cascade; rostrocaudal neural tube patterning

Disease: Mental Retardation, X-linked, Syndromic, Hedera Type; Parkinsonism With Spasticity, X-linked