Mouse Renin receptor ELISA Kit | Atp6ap2 elisa kit

Mouse Renin receptor ELISA Kit

Cat. Num. AAA760581

• Gene Names: ATP6AP2; PRR; M8-9; MRXE; RENR; XMRE; XPDS; HT028; MRXSH; ELDF10; ATP6IP2; MSTP009; APT6M8-9; ATP6M8-9
• Reactivity: Mouse
• Synonyms: Renin receptor; Mouse Renin receptor ELISA Kit; Atp6ap2/ATP6IP2/ATP6M8-9/CAPER/ELDF10/HT028/M8-9/MRXE/MSTP009/N14F/XMRE/ATPase H (+)-transporting lysosomal accessory protein 2/ATPase H (+)-transporting lysosomal-interacting protein 2/Embryonic liver differentiation factor 10/ER-localized type I transmembrane adaptor/vacuolar proton ATP synthase membrane sector associated protein M8-9/V-ATPase M8.9 subunit; Atp6ap2 elisa kit

• Reactivity: Mouse
• Sequence Length: 350

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Atp6ap2 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Atp6ap2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Atp6ap2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Atp6ap2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Atp6ap2 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 15011918
• NCBI GeneID: 10159
• NCBI Accession #: NP_005756.2
• NCBI GenBank Nucleotide #: NM_005765.2
• Molecular Weight: 35,612 Da
• NCBI Official Full Name: renin receptor
• NCBI Official Synonym Full Names: ATPase H+ transporting accessory protein 2
• NCBI Official Symbol: ATP6AP2
• NCBI Official Synonym Symbols: PRR; M8-9; MRXE; RENR; XMRE; XPDS; HT028; MRXSH; ELDF10; ATP6IP2; MSTP009; APT6M8-9; ATP6M8-9
• NCBI Protein Information: renin receptor
• UniProt Protein Name: Renin receptor
• Protein Family: Renin receptor
• UniProt Gene Name: ATP6AP2
• UniProt Synonym Gene Names: ATP6IP2; CAPER; ELDF10; ATP6M8-9; V-ATPase M8.9 subunit
• UniProt Entry Name: RENR_HUMAN

NCBI Description

This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases. [provided by RefSeq, Jul 2008]

Uniprot Description

ATP6AP2: Functions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS). Defects in ATP6AP2 are a cause of mental retardation X- linked with epilepsy (MRXE). MRXE is a syndromic mental retardation. Patients manifest mild to moderate mental retardation associated with epilepsy, delays in motor milestones and speech acquisition in infancy.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp11.4

Cellular Component: external side of plasma membrane; integral to membrane; neuron projection; plasma membrane

Molecular Function: aspartic-type endopeptidase activity; enzyme binding; protein binding; receptor activity

Biological Process: angiotensin maturation; cellular protein metabolic process; eye pigmentation; positive regulation of transforming growth factor-beta1 production; positive regulation of Wnt receptor signaling pathway; regulation of MAPKKK cascade; rostrocaudal neural tube patterning

Disease: Mental Retardation, X-linked, Syndromic, Hedera Type; Parkinsonism With Spasticity, X-linked