Typical Testing Data/Standard Curve (for reference only)
Indole 3 Acetic Acid (IAA) ELISA Kit | IAA elisa kit
General Indole 3 Acetic Acid (IAA) Mini Samples ELISA Kit
Reactivity
Pan-species (General)
Synonyms
Indole 3 Acetic Acid (IAA); General Indole 3 Acetic Acid (IAA) Mini Samples ELISA Kit; IA-A; Indolylacetic Acid; Indoleacetic Acid; Heteroauxin; IAA elisa kit
Reactivity
Pan-species (General)
Specificity
This assay has high sensitivity and excellent specificity for detection of IAA. No significant cross-reactivity or interference between IAA and analogues was observed.
Sequence Length
825
Samples
Tissue or cell culture supernates
Assay Type
Quantitative Competitive
Detection Range
320-200,000pg/mL
Sensitivity
< 112pg/mL
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level IAA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level IAA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for IAA elisa kit
Intended Uses: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of IAA in tissue or cell culture supernates.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to IAA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled IAA and unlabeled IAA (Standards or samples) with the pre-coated antibody specific to IAA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of IAA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of IAA in the sample.
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to IAA has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled IAA and unlabeled IAA (Standards or samples) with the pre-coated antibody specific to IAA. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of IAA in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of IAA in the sample.
Product Categories/Family for IAA elisa kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
88,759 Da
NCBI Official Full Name
indole-3-acetic acid
UniProt Protein Name
Auxin-responsive protein
UniProt Gene Name
MpIAA
Uniprot Description
Aux/IAA proteins are short-lived transcriptional factors that function as repressors of early auxin response genes at low auxin concentrations.
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Product Notes
The Pan-species (General) IAA mpiaa (Catalog #AAA2087559) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2087559 ELISA Kit recognizes Pan-species (General) IAA. It is sometimes possible for the material contained within the vial of "Indole 3 Acetic Acid (IAA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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