Western Blot (WB)
(Western Blot: Sample: Human Serum; Primary Ab: 3ug/ml Mouse Anti-Human C5a Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Mouse anti-Human Complement Component 5a (C5a) Monoclonal Antibody | anti-C5a antibody
Monoclonal Antibody to Complement Component 5a (C5a)
Immunocytochemistry in formalin fixed cells: 5-30ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml
Immunohistochemistry in paraffin section: 5-30ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Human Serum; Primary Ab: 3ug/ml Mouse Anti-Human C5a Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Human Serum; Primary Ab: 3ug/ml Mouse Anti-Human C5a Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant C5a, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant C5a, Human.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreatic cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreatic cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreatic cancer Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Human Pancreatic cancer Tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a component of the complement system, a part of the innate immune system that plays an important role in inflammation, host homeostasis, and host defense against pathogens. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the C5 alpha chain, C5 beta chain, C5a anaphylatoxin and C5b. The C5 protein is comprised of the C5 alpha and beta chains, which are linked by a disulfide bridge. Cleavage of the alpha chain by a convertase enzyme results in the formation of the C5a anaphylatoxin, which possesses potent spasmogenic and chemotactic activity, and the C5b macromolecular cleavage product, a subunit of the membrane attack complex (MAC). Mutations in this gene cause complement component 5 deficiency, a disease characterized by recurrent bacterial infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2015]
Uniprot Description
C5: Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. Defects in C5 are the cause of complement component 5 deficiency (C5D). A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis. An association study of C5 haplotypes and genotypes in individuals with chronic hepatitis C virus infection shows that individuals homozygous for the C5_1 haplotype have a significantly higher stage of liver fibrosis than individuals carrying at least 1 other allele (PubMed:15995705).
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9q33.2
Cellular Component: extracellular region; extracellular space; membrane attack complex
Molecular Function: chemokine activity; endopeptidase inhibitor activity; protein binding; receptor binding
Biological Process: activation of MAPK activity; cell surface receptor signaling pathway; chemotaxis; complement activation; complement activation, alternative pathway; complement activation, classical pathway; cytolysis; G-protein coupled receptor signaling pathway; in utero embryonic development; inflammatory response; positive regulation of angiogenesis; regulation of complement activation; response to stress
Disease: Complement Component 5 Deficiency; Eculizumab, Poor Response To
Research Articles on C5a
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Product Notes
The C5a c5 (Catalog #AAA2085839) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Complement Component 5a (C5a) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Complement Component 5a (C5a) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP). Western Blot: 0.5-5ug/ml Immunocytochemistry in formalin fixed cells: 5-30ug/ml Immunohistochemistry in formalin fixed frozen section: 5-30ug/ml Immunohistochemistry in paraffin section: 5-30ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml. Researchers should empirically determine the suitability of the C5a c5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Complement Component 5a (C5a), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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