CHI3L1 recombinant protein

CHI3L1, 22-383aa, Human, His-tag, Baculovirus

Cat. Num. AAA206411

• Gene Names: CHI3L1; GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: CHI3L1; 22-383aa; Human; His-tag; Baculovirus; Chitinase-3-like protein 1; ASRT7; CGP-39; GP-39; GP39; HC-gp39; hCGP-39; HCGP-3P; YKL-40; YYL-40; 39 kDa synovial protein; Cartilage glycoprotein 39 CGP-39; CGP39; CH3L1_HUMAN; chitinase chitinase 3 like 1; chitinase 3 like 1 (cartilage glycoprotein 39); Chitinase 3 like protein 1 precursor Chitinase-3-like protein 1; Chondrocyte protein YKL40 GP 39; HC gp39; HCGP 3P; hCGP-39 HCgp39; YKL 40; YKL40; YYL 40; CHI3L1 recombinant protein

• Host: Baculovirus
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol, 1mM DTT
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: YKLVCYYTSW SQYREGDGSC FPDALDRFLC THIIYSFANI SNDHIDTWEW NDVTLYGMLN TLKNRNPNLK TLLSVGGWNF GSQRFSKIAS NTQSRRTFIK SVPPFLRTHG FDGLDLAWLY PGRGDKQHFT TLIKEMKAEF IKEAQPGKKQ LLLSAALSAG KVTIDSSYDI AKISQHLDFI SIMTYDFHGA WRGTTGHHSP LFRGQEDASP DRFSNTDYAV GYMLRLGAPA SKLVMGIPTF GRSFTLASSE TGVGAPISGP GIPGRFTKEA GTLAYYEICD FLRGATVHRI LGQQVPYATK GNQWVGYDDQ ESVKSKVQYL KDRQLAGAMV WALDLDDFQG SFCGQDLRFP LTNAIKDALA ATLEHHHHHH
• Sequence Length: 383

• Applicable Applications for CHI3L1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for CHI3L1 recombinant protein

CHI3L1, also known as chitinase-3-like protein 1, is a member of the glycosyl hydrolase 18 family. This protein can bind heparins, probably as heparin sulfate. It has been found to enhance cell adhesion and promote cell signaling, proliferation and tumor angiogenesis. Elevated serum CHI3L1 levels occur in some conditions characterized by inflammation and connective tissue remodeling such as arthritis, chronic obstructive pulmonary disease, diabetes, cardiovascular disease, inflammatory bowel disease, and liver cirrhosis. Recombinant human CHI3L1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques

Product Categories/Family for CHI3L1 recombinant protein

Signal Transduction; Cytoskeleton

References

Hakala BE., et al. (1993) J Biol Chem 268:25803-25810.; Eurich K., et al. (2009) World J Gastroenterol. 15:5249-5259.;

NCBI and Uniprot Product Information

• NCBI GI #: 144226251
• NCBI GeneID: 1116
• NCBI Accession #: NP_001267
• NCBI GenBank Nucleotide #: NM_001276.2
• UniProt Accession #: P36222; P30923; Q8IVA4; Q96HI7; B2R7B0
• Molecular Weight: 41.4kDa (370aa) 40-57KDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: chitinase-3-like protein 1
• NCBI Official Synonym Full Names: chitinase 3 like 1
• NCBI Official Symbol: CHI3L1
• NCBI Official Synonym Symbols: GP39; ASRT7; GP-39; YKL40; CGP-39; YKL-40; YYL-40; HC-gp39; HCGP-3P; hCGP-39
• NCBI Protein Information: chitinase-3-like protein 1
• UniProt Protein Name: Chitinase-3-like protein 1
• Protein Family: Chitinase-3-like protein
• UniProt Gene Name: CHI3L1
• UniProt Synonym Gene Names: CGP-39; GP-39; hCGP-39

NCBI Description

Chitinases catalyze the hydrolysis of chitin, which is an abundant glycopolymer found in insect exoskeletons and fungal cell walls. The glycoside hydrolase 18 family of chitinases includes eight human family members. This gene encodes a glycoprotein member of the glycosyl hydrolase 18 family. The protein lacks chitinase activity and is secreted by activated macrophages, chondrocytes, neutrophils and synovial cells. The protein is thought to play a role in the process of inflammation and tissue remodeling. [provided by RefSeq, Sep 2009]

Uniprot Description

Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung.