GBA recombinant protein

GBA, 40-536aa, Human, His tag, Baculovirus

Cat. Num. AAA206272

• Gene Names: GBA; GCB; GBA1; GLUC
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: GBA; 40-536aa; Human; His tag; Baculovirus; Glucosylceramidase isoform 1; GBA1; GCB; GLUC; GBA recombinant protein

• Host: Baculovirus
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. In phosphate buffered saline (pH 7.4) containing 10% glycerol.
• Concentration: 0.5 mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence: ARPCIPKSFG YSSVVCVCNA TYCDSFDPPT FPALGTFSRY ESTRSGRRME LSMGPIQANH TGTGLLLTLQ PEQKFQKVKG FGGAMTDAAA LNILALSPPA QNLLLKSYFS EEGIGYNIIR VPMASCDFSI RTYTYADTPD DFQLHNFSLP EEDTKLKIPL IHRALQLAQR PVSLLASPWT SPTWLKTNGA VNGKGSLKGQ PGDIYHQTWA RYFVKFLDAY AEHKLQFWAV TAENEPSAGL LSGYPFQCLG FTPEHQRDFI ARDLGPTLAN STHHNVRLLM LDDQRLLLPH WAKVVLTDPE AAKYVHGIAV HWYLDFLAPA KATLGETHRL FPNTMLFASE ACVGSKFWEQ SVRLGSWDRG MQYSHSIITN LLYHVVGWTD WNLALNPEGG PNWVRNFVDS PIIVDITKDT FYKQPMFYHL GHFSKFIPEG SQRVGLVASQ KNDLDAVALM HPDGSAVVVV LNRSSKDVPL TIKDPAVGFL ETISPGYSIH TYLWRRQHHH HHH
• Sequence Length: 536

• Applicable Applications for GBA recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Endotoxin Level: < 1.0 EU per 1 microgram of protein (determined by LAL method)
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for GBA recombinant protein

GBA, also known as glucosylceramidase isoform 1, is a lysosomal enzyme that presumably requires a signal peptide for transport across the membrane of the rough endoplasmic reticulum and glycosylation for transport into lysosomes. Gaucher disease is due to a deficiency in the activity of the enzyme glucocerebrosidase. Recombinant human GBA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for GBA recombinant protein

Neuroscience; Enzymes & Proteases

References

Sorge JA., et al. (1987) Am J Hum Genet. 41:1016-1024.; Salvioli R., et al. (2000) FEBS Lett. 472:17-21.

NCBI and Uniprot Product Information

• NCBI GI #: 54607043
• NCBI GeneID: 2629
• NCBI Accession #: NP_000148
• NCBI GenBank Nucleotide #: NM_000157.3
• UniProt Accession #: P04062; Q16545; Q4VX22; Q6I9R6; Q9UMJ8; A8K796; B7Z5G2; B7Z6S1; J3KQG4; J3KQK9
• Molecular Weight: 56.4kDa (503aa) 50-70kDa (SDS-PAGE under reducing conditions)
• NCBI Official Full Name: glucosylceramidase isoform 1
• NCBI Official Synonym Full Names: glucosylceramidase beta
• NCBI Official Symbol: GBA
• NCBI Official Synonym Symbols: GCB; GBA1; GLUC
• NCBI Protein Information: glucosylceramidase
• UniProt Protein Name: Glucosylceramidase
• Protein Family: Glucosylceramidase
• UniProt Gene Name: GBA
• UniProt Synonym Gene Names: GC; GLUC; Beta-GC

NCBI Description

This gene encodes a lysosomal membrane protein that cleaves the beta-glucosidic linkage of glycosylceramide, an intermediate in glycolipid metabolism. Mutations in this gene cause Gaucher disease, a lysosomal storage disease characterized by an accumulation of glucocerebrosides. A related pseudogene is approximately 12 kb downstream of this gene on chromosome 1. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2010]