Mouse anti-Human FUCA2 Monoclonal Antibody | anti-FUCA2 antibody

FUCA2 (FUCA2, Plasma alpha-L-fucosidase, Alpha-L-fucoside fucohydrolase 2) (AP)

Cat. Num. AAA6120828

• Gene Names: FUCA2; dJ20N2.5
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: FUCA2; Monoclonal Antibody; FUCA2 (FUCA2; Plasma alpha-L-fucosidase; Alpha-L-fucoside fucohydrolase 2) (AP); Alpha-L-fucoside fucohydrolase 2; anti-FUCA2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 234CT20.2.1
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-FUCA2 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: ELISA: 1:1000; WB: 1:100-250; Applications are based on unconjugated antibody.
• Immunogen: FUCA2 Mab is generated from mouse immunized with FUCA2 recombinant protein.
• Conjugate: AP
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C with the addition of glycerol. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive.

Western Blot (WB)

(FUCA2 western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the FUCA2 antibody detected the FUCA2 protein (arrow).)

Related Product Information for anti-FUCA2 antibody

This gene encodes a plasma alpha-L-fucosidase, which represents 10-20% of the total cellular fucosidase activity. The protein is a member of the glycosyl hydrolase 29 family, and catalyzes the hydrolysis of the alpha-1,6-linked fucose joined to the reducing-end N-acetylglucosamine of the carbohydrate moieties of glycoproteins. This enzyme is essential for Helicobacter pylori adhesion to human gastric cancer cells.

Product Categories/Family for anti-FUCA2 antibody

Antibodies; Cancer Markers

References

Liu, T.W., et al. Proc. Natl. Acad. Sci. U.S.A. 106(34):14581-14586(2009) Shah, M., et al. Cancer 113(2):336-346(2008) Lamesch, P., et al. Genomics 89(3):307-315(2007) Mungall, A.J., et al. Nature 425(6960):805-811(2003) Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)

NCBI and Uniprot Product Information

• NCBI GI #: 40068512
• NCBI GeneID: 2519
• NCBI Accession #: NP_114409.2
• NCBI GenBank Nucleotide #: NM_032020.4
• UniProt Accession #: Q9BTY2; Q7Z6V1; Q7Z6Y2; Q8NBK4; E9PEB6
• Molecular Weight: 19,835 Da
• NCBI Official Full Name: plasma alpha-L-fucosidase
• NCBI Official Synonym Full Names: fucosidase, alpha-L- 2, plasma
• NCBI Official Symbol: FUCA2
• NCBI Official Synonym Symbols: dJ20N2.5
• NCBI Protein Information: plasma alpha-L-fucosidase
• UniProt Protein Name: Plasma alpha-L-fucosidase
• Protein Family: Plasma alpha-L-fucosidase
• UniProt Gene Name: FUCA2
• UniProt Synonym Gene Names: Alpha-L-fucosidase 2
• UniProt Entry Name: FUCO2_HUMAN

Uniprot Description

FUCA2: Alpha-L-fucosidase is responsible for hydrolyzing the alpha-1,6-linked fucose joined to the reducing-end N- acetylglucosamine of the carbohydrate moieties of glycoproteins. Belongs to the glycosyl hydrolase 29 family.

Protein type: Secreted; EC 3.2.1.51; Glycan Metabolism - other glycan degradation; Hydrolase; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6q24

Cellular Component: extracellular space; lysosome

Molecular Function: alpha-L-fucosidase activity; fucose binding

Biological Process: fucose metabolic process; glycoside catabolic process; response to bacterium