SDS-Page
ZFAND5 recombinant protein
ZFAND5, 1-213aa, Human, His tag, E Coli
Gene Names
ZFAND5; ZA20D2; ZNF216; ZFAND5A
Applications
SDS-Page
Purity
> 85% by SDS-PAGE
Synonyms
ZFAND5; 1-213aa; Human; His tag; E Coli; AN1-type zinc finger protein 5; ZA20D2; ZFAND5A; ZNF216; ZFAND5 recombinant protein
Host
E Coli
Purity/Purification
> 85% by SDS-PAGE
Form/Format
Liquid. In 20mM Phosphate buffer saline (pH 8.0) containing 30% glycerol, 1mM DTT
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAQETNQ TPGPMLCSTG CGFYGNPRTN GMCSVCYKEH LQRQQNSGRM SPMGTASGSN SPTSDSASVQ RADTSLNNCE GAAGSTSEKS RNVPVAALPV TQQMTEMSIS REDKITTPKT EVSEPVVTQP SPSVSQPSTS QSEEKAPELP KPKKNRCFMC RKKVGLTGFD CRCGNLFCGL HRYSDKHNCP YDYKAEAAAK IRKENPVVVA EKIQRI
Sequence Length
213
Applicable Applications for ZFAND5 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for ZFAND5 recombinant protein
ZFAND5 is involved in protein degradation via the ubiquitin-proteasome system. This protein may act by anchoring ubiquitinated proteins to the proteasome and plays a role in ubiquitin-mediated protein degradation during muscle atrophy. ZFAND5 plays a role in the regulation of NF-kappa-B activation and apoptosis and inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. It inhibits also tumor necrosis factor (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Recombinant human ZFAND5 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for ZFAND5 recombinant protein
References
Huang J., et al. (2004) J. Biol. Chem. 279:16847-16853.; Gauci S., et al. (2009) Anal. Chem. 81:4493-4501.;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25.5 kDa (236aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Official Full Name
AN1-type zinc finger protein 5
NCBI Official Synonym Full Names
zinc finger AN1-type containing 5
NCBI Official Symbol
ZFAND5
NCBI Official Synonym Symbols
ZA20D2; ZNF216; ZFAND5A
NCBI Protein Information
AN1-type zinc finger protein 5
UniProt Protein Name
AN1-type zinc finger protein 5
Protein Family
UniProt Gene Name
ZFAND5
UniProt Synonym Gene Names
ZA20D2; ZNF216
Uniprot Description
Involved in protein degradation via the ubiquitin-proteasome system. May act by anchoring ubiquitinated proteins to the proteasome. Plays a role in ubiquitin-mediated protein degradation during muscle atrophy. Plays a role in the regulation of NF-kappa-B activation and apoptosis. Inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. Inhibits also tumor necrosis factor (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Overexpression sensitizes cells to TNF-induced apoptosis. Is a potent inhibitory factor for osteoclast differentiation.
Research Articles on ZFAND5
1. Zfand5 can enhance ARE-containing mRNA stability by competing with tristetraprolin for mRNA binding.
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Product Notes
The ZFAND5 zfand5 (Catalog #AAA206074) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's ZFAND5 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the ZFAND5 zfand5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMAQETNQ TPGPMLCSTG CGFYGNPRTN GMCSVCYKEH LQRQQNSGRM SPMGTASGSN SPTSDSASVQ RADTSLNNCE GAAGSTSEKS RNVPVAALPV TQQMTEMSIS REDKITTPKT EVSEPVVTQP SPSVSQPSTS QSEEKAPELP KPKKNRCFMC RKKVGLTGFD CRCGNLFCGL HRYSDKHNCP YDYKAEAAAK IRKENPVVVA EKIQRI. It is sometimes possible for the material contained within the vial of "ZFAND5, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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