N6AMT1 recombinant protein

N6AMT1, 1-214aa, Human, His tag, E Coli

Cat. Num. AAA205993

• Gene Names: N6AMT1; MTQ2; HEMK2; N6AMT; PRED28; C21orf127; m.HsaHemK2P
• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: N6AMT1; 1-214aa; Human; His tag; E Coli; HemK methyltransferase family member 2; C21orf127; HEMK2; MTQ2; N6AMT; PRED28; N6AMT1 recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid, In Phosphate buffered saline (pH 7.4) containing 20% glycerol, 1mM DTT
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGENFA TPFHGHVGRG AFSDVYEPAE DTFLLLNALE AAAAELAGVE ICLEVGSGSG VVSAFLASMI GPQALYMCTD INPEAAACTL ETARCNKVHI QPVITDLVKG LLPRLTEKVD LLVFNPPYVV TPPQEVGSHG IEAAWAGGRN GREVMDRFFP LVPDLLSPRG LFYLVTIKEN NPEEILKIMK TKGLQGTTAL SRQAGQETLS VLKFTKS
• Sequence Length: 214

• Applicable Applications for N6AMT1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for N6AMT1 recombinant protein

N6AMT1 is a member of methyltransferase family, may be involved in the methylation of release factor I during translation termination. This protein is also involved in converting the arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternate splicing results in multiple transcript variants. Recombinant human N6ATM1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for N6AMT1 recombinant protein

Epigenomics (Transcription & Translation)

References

Figaro S., et al. (2008) FEBS Lett. 582:2352-2356; Ren X., et al. (2011) Environ. Health Perspect. 119:771-777;

NCBI and Uniprot Product Information

• NCBI GI #: 669174630
• NCBI GeneID: 29104
• NCBI Accession #: NP_037372
• NCBI GenBank Nucleotide #: NM_013240.5
• UniProt Accession #: Q9Y5N5; Q96F73; B2RA97
• Molecular Weight: 25.3 kDa (237aa) confirmed by MALDI-TOF
• NCBI Official Full Name: hemK methyltransferase family member 2 isoform 1
• NCBI Official Synonym Full Names: N-6 adenine-specific DNA methyltransferase 1
• NCBI Official Symbol: N6AMT1
• NCBI Official Synonym Symbols: MTQ2; HEMK2; N6AMT; PRED28; C21orf127; m.HsaHemK2P
• NCBI Protein Information: hemK methyltransferase family member 2
• UniProt Protein Name: HemK methyltransferase family member 2
• Protein Family: HemK methyltransferase family
• UniProt Gene Name: N6AMT1
• UniProt Synonym Gene Names: C21orf127; HEMK2

NCBI Description

This gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing pf this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 11. [provided by RefSeq, Jul 2014]

Uniprot Description

Heterodimeric methyltransferase that catalyzes N5-methylation of ETF1 on 'Gln-185', using S-adenosyl L-methionine as methyl donor. ETF1 needs to be complexed to ERF3 in its GTP-bound form to be efficiently methylated. May play a role in the modulation of arsenic-induced toxicity. May be involved in the conversion of monomethylarsonous acid (3+) into the less toxic dimethylarsonic acid.