MRPL2 recombinant protein

MRPL2, 84-202aa, Human, His tag, E Coli

Cat. Num. AAA205897

• Gene Names: MRPL2; CGI-22; RPML14; MRP-L14
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: MRPL2; 84-202aa; Human; His tag; E Coli; 39S ribosomal protein L2 mitochondrial; 39S ribosomal protein L2; mitochondrial; CGI-22; MRP-L14; RPML14; MRPL2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Phosphate buffer (pH 8.0) containing 1mM EDTA, 50% glycerol, 2mM DTT.
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSGRDHTGR IRVHGIGGGH KQRYRMIDFL RFRPEETKSG PFEEKVIQVR YDPCRSADIA LVAGGSRKRW IIATENMQAG DTILNSNHIG RMAVAAREGD AHPLGALPVG TLINNVESEP GR
• Sequence Length: 305

• Applicable Applications for MRPL2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for MRPL2 recombinant protein

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. MRPL2 is a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. Recombinant human MRPL2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for MRPL2 recombinant protein

Epigenomics (Transcription & Translation)

References

O'Brien TW, Fiesler SE, et al. (1999). J Biol Chem. 274 (51):36043-51.; Kenmochi N, Suzuki T, et al. (2001). Genomics. 77 (1-2):65-70.;

NCBI and Uniprot Product Information

• NCBI GI #: 21265070
• NCBI GeneID: 51069
• NCBI Accession #: NP_057034
• NCBI GenBank Nucleotide #: NM_015950.4
• UniProt Accession #: Q5T653; Q8WUL1; Q96Q56; Q9Y311; B2RC56
• Molecular Weight: 15.5 kDa (142aa) confirmed by MALDI-TOF.
• NCBI Official Full Name: 39S ribosomal protein L2, mitochondrial isoform 1
• NCBI Official Synonym Full Names: mitochondrial ribosomal protein L2
• NCBI Official Symbol: MRPL2
• NCBI Official Synonym Symbols: CGI-22; RPML14; MRP-L14
• NCBI Protein Information: 39S ribosomal protein L2, mitochondrial
• UniProt Protein Name: 39S ribosomal protein L2, mitochondrial
• Protein Family: 39S ribosomal protein
• UniProt Gene Name: MRPL2
• UniProt Synonym Gene Names: L2mt; MRP-L2

NCBI Description

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2014]