PuRB recombinant protein

PuRB, 1-312aa, Human, His tag, E Coli

Cat. Num. AAA205607

• Gene Names: PURB; PURBETA
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: PuRB; 1-312aa; Human; His tag; E Coli; Transcriptional activator protein Pur-beta; Purine-rich element binding protein B; PuRBETA; PuRB recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMADGDSG SERGGGGGPC GFQPASRGGG EQETQELASK RLDIQNKRFY LDVKQNAKGR FLKIAEVGAG GSKSRLTLSM AVAAEFRDSL GDFIEHYAQL GPSSPEQLAA GAEEGGGPRR ALKSEFLVRE NRKYYLDLKE NQRGRFLRIR QTVNRGGGGF GAGPGPGGLQ SGQTIALPAQ GLIEFRDALA KLIDDYGGED DELAGGPGGG AGGPGGGLYG ELPEGTSITV DSKRFFFDVG CNKYGVFLRV SEVKPSYRNA ITVPFKAWGK FGGAFCRYAD EMKEIQERQR DKLYERRGGG SGGGEESEGE EVDED
• Sequence Length: 312

• Applicable Applications for PuRB recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for PuRB recombinant protein

PuRB is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PuR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. Recombinant human PuRB protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for PuRB recombinant protein

Epigenomics (Transcription & Translation)

References

Bergemann A.D., Ma Z.-W., et al. (1992) Mol. Cell. Biol. 12:5673-5682

NCBI and Uniprot Product Information

• NCBI GI #: 15147219
• NCBI GeneID: 5814
• NCBI Accession #: NP_150093
• NCBI GenBank Nucleotide #: NM_033224.4
• UniProt Accession #: Q96QR8; A4D2L7
• Molecular Weight: 35.6kDa (335aa) confirmed by MALDI-TOF
• NCBI Official Full Name: transcriptional activator protein Pur-beta
• NCBI Official Synonym Full Names: purine rich element binding protein B
• NCBI Official Symbol: PURB
• NCBI Official Synonym Symbols: PURBETA
• NCBI Protein Information: transcriptional activator protein Pur-beta
• UniProt Protein Name: Transcriptional activator protein Pur-beta
• Protein Family: Transcriptional activator protein
• UniProt Gene Name: PURB

NCBI Description

This gene product is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PUR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. [provided by RefSeq, Jul 2008]

Uniprot Description

Has capacity to bind repeated elements in single-stranded DNA such as the purine-rich single strand of the PUR element located upstream of the MYC gene. Plays a role in the control of vascular smooth muscle (VSM) alpha-actin gene transcription as repressor in myoblasts and fibroblasts. Participates in transcriptional and translational regulation of alpha-MHC expression in cardiac myocytes by binding to the purine-rich negative regulatory (PNR) element. Modulates constitutive liver galectin-3 gene transcription by binding to its promoter. May play a role in the dendritic transport of a subset of mRNAs ().