RPRD1A recombinant protein

RPRD1A, 1-312aa, Human, His tag, E Coli

Cat. Num. AAA205573

• Gene Names: RPRD1A; P15RS; HsT3101
• Applications: SDS-Page
• Purity: > 95% by SDS-PAGE
• Synonyms: RPRD1A; 1-312aa; Human; His tag; E Coli; Regulation of nuclear pre-mRNA domain-containing protein 1A; Regulation of nuclear pre-mRNA domain containing 1A; HsT3101; P15RS; RPRD1A recombinant protein

• Host: E Coli
• Purity/Purification: > 95% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
• Concentration: 0.5 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSEFMSAFS EAALEKKLSE LSNSQQSVQT LSLWLIHHRK HSRPIVTVWE RELRKAKPNR KLTFLYLAND VIQNSKRKGP EFTKDFAPVI VEAFKHVSSE TDESCKKHLG RVLSIWEERS VYENDVLEQL KQALYGDKKP RKRTYEQIKV DENENCSSLG SPSEPPQTLD LVRALQDLEN AASGDAAVHQ RIASLPVEVQ EVSLLDKITD KESGERLSKM VEDACMLLAD YNGRLAAEID DRKQLTRMLA DFLRCQKEAL AEKEHKLEEY KRKLARVSLV RKELRSRIQS LPDLSRLPNV TGSHMHLPFA GDIYSED
• Sequence Length: 312

• Applicable Applications for RPRD1A recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for RPRD1A recombinant protein

RPRD1A is upregulated in cells overexpressing cyclin-dependent kinase inhibitor p15 (INK4b) and may have a role in cell cycle regulation Recombinant human RPRD1A protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for RPRD1A recombinant protein

Cell Cycle

References

Liu J., Liu H., Zhang X., et al. (2002) Biochem. Biophys. Res. Commun. 299:880-885; Ni Z., Olsen J.B., et al. (2011) Transcription 2:237-242;

NCBI and Uniprot Product Information

• NCBI GI #: 21361709
• NCBI GeneID: 55197
• NCBI Accession #: NP_060640
• NCBI GenBank Nucleotide #: NM_018170.4
• UniProt Accession #: Q96P16; Q7Z5G8; Q96FY9; Q9NVL4; A8KA42; B2RBA3
• Molecular Weight: 38.4kDa (337aa) confirmed by MALDI-TOF
• NCBI Official Full Name: regulation of nuclear pre-mRNA domain-containing protein 1A isoform 1
• NCBI Official Synonym Full Names: regulation of nuclear pre-mRNA domain containing 1A
• NCBI Official Symbol: RPRD1A
• NCBI Official Synonym Symbols: P15RS; HsT3101
• NCBI Protein Information: regulation of nuclear pre-mRNA domain-containing protein 1A
• UniProt Protein Name: Regulation of nuclear pre-mRNA domain-containing protein 1A
• Protein Family: Regulation of nuclear pre-mRNA domain-containing protein
• UniProt Gene Name: RPRD1A
• UniProt Synonym Gene Names: P15RS

NCBI Description

This gene encodes a cell-cycle and transcription regulatory protein. The encoded protein interacts with the cell cycle inhibitor cyclin-dependent kinase 4 inhibitor B and may function as a negative regulator of G(1)/S phase progression. This protein also forms homo- and hetrodimers with the protein, regulation of nuclear pre-mRNA domain-containing protein 1B, to form a scaffold that interacts with the C-terminal domain of RNA polymerase II subunit B1 and regulates several aspects of transcription. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 16. [provided by RefSeq, Dec 2014]

Uniprot Description

Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. May act as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle.